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NDT Advance Access published online on June 16, 2008
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfn268
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© The Author [2008]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org


The protective role of uteroglobin through the modulation of tissue transglutaminase in the experimental crescentic glomerulonephritis

Seung Hee Yang1,2, Sung Joon Shin3, Ji Eun Oh4, Ji Zhe Jin1, Nam Hyun Chung2, Chun Soo Lim1, Suhnggwon Kim1 and Yon Su Kim1

1 Department of Internal Medicine, Seoul National University College of Medicine, Kidney Research Institute 2 Korea University College of Life Science and Biotechnology 3 Department of Internal Medicine, Dongguk University College of Medicine 4 Department of Internal Medicine, Hallym University College of Medicine, Seoul, Korea

Correspondence and offprint requests to: Yon Su Kim, Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul, 110-744, Korea. Tel: +82-2-2072-2264; Fax: +82-2-745-2264; E-mail: yonsukim{at}snu.ac.kr



  Abstract

Background and methods. Tissue transglutaminase (tTG) may induce pro-inflammatory cytokines and produce irreversible end-products, thus promoting renal scarring. It has recently been confirmed that the crescent formation in murine experimental crescentic glomerulonephritis (ecGN) has been inhibited by the administration of recombinant uteroglobin (rUG). However, the ability of UG on tTG modulation has not been thoroughly assessed. In this study, we investigated the feasible protective role of UG in murine ecGN through the modulation of tTG and TGF-β1 expressions. ecGN was induced by the administration of anti-GBM Ab into C57BL/6 mice.

Results. Both proteinuria and BUN levels were distinctively lower in rUG-treated mice compared to those of disease control mice. Glomerular injuries such as mesangial proliferation, matrix production and crescent formation were lessened with the rUG treatment, and these findings were parallel with the attenuated expression of tTG and TGF-β1. tTG and TGF-β1 were expressed mainly on mesangial areas by the induction of ecGN and rUG treatment markedly attenuated the expressions of these proteins in glomeruli without spatial changes. With the addition of LPS to mesangial cells, the expressions of tTG and TGF-β1 were up-regulated, whilst the addition of cysteamine, tTG inhibitor, attenuated the expression of tTG and TGF-β1 as well as the cellular proliferation which was further induced by LPS.

Conclusion. We demonstrate for the first time that rUG is able to attenuate the renal injury through the modulation of expressions of tTG and TGF-β1 in ecGN and further suggest a wide range of feasible molecular targets to reduce the severity of human glomerulonephritis.

Keywords: experimental crescentic glomerulonephritis; tissue transglutaminase; uteroglobin

Received for publication: 24.12.07
Accepted in revised form: 17. 4.08


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