Skip Navigation



NDT Advance Access published online on September 27, 2006
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfl519
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
22/1/128    most recent
gfl519v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Verkade, M. A.
Right arrow Articles by Betjes, M. G. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verkade, M. A.
Right arrow Articles by Betjes, M. G. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received June 23, 2006
Accepted August 7, 2006

Original Article

Functional impairment of monocyte-derived dendritic cells in patients with severe chronic kidney disease

Martijn Anton Verkade 1, Corné Johan van Druningen 1, Leonard Maria Bernardus Vaessen 1, Dennis Adriaan Hesselink 1, Willem Weimar 1, and Michiel Gerardus Henricus Betjes 1 *

1 Department of Nephrology, Erasmus Medical Center, Rotterdam, The Netherlands

* To whom correspondence should be addressed.
Michiel Gerardus Henricus Betjes, E-mail: M.G.H.Betjes{at}erasmusmc.nl



  Abstract

Background. Dendritic cells (DCs) are antigen-presenting cells that are pivotal for the initiation of the primary immune response. Patients with chronic kidney disease (CKD) with or without chronic intermittent haemodialysis (CIHD) show an impaired immune response. Dysfunction of DCs may underlie this phenomenon.

Methods. In this study, several different functions of monocyte-derived DCs (moDC) of patients with CKD class IV-V (glomerular filtration rate <30 ml/min) and patients on CIHD were studied in vitro and compared with age- and sex-matched healthy volunteers.

Results. We demonstrate that, independent of the maturation stimulus used, mature moDC from both groups of patients did not acquire the same level of terminal differentiation as moDC from controls, as shown by analysis of cell surface markers and the relative high macropinocytosis activity of moDC. The stimulation of allogeneic T-cells by immature moDC and mature moDC did not differ between patients and controls. However, in the presence of immature moDC or antigen-loaded maturated moDC from patients, less proliferation of autologous T-cells was observed in response to recall antigens. There was no difference between moDC from controls and patients in their ability to activate naive T-cells and to differentiate them into Th1 and Th2 cells.

Conclusions. These results show that the terminal differentiation of moDC in patients with severe CKD is impaired. This impairment is not restricted to one maturation stimulus and is independent of treatment with haemodialysis.

Keywords: chronic kidney disease; dendritic cells; immune deficiency; terminal differentiation.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
S. Agrawal, P. Gollapudi, R. Elahimehr, M. V. Pahl, and N. D. Vaziri
Effects of end-stage renal disease and haemodialysis on dendritic cell subsets and basal and LPS-stimulated cytokine production
Nephrol. Dial. Transplant., November 9, 2009; (2009) gfp580v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
N. H. R. Litjens, M. Huisman, D. Hijdra, B. M. N. Lambrecht, K. J. Stittelaar, and M. G. H. Betjes
IL-2 Producing Memory CD4+ T Lymphocytes Are Closely Associated with the Generation of IgG-Secreting Plasma Cells
J. Immunol., September 1, 2008; 181(5): 3665 - 3673.
[Abstract] [Full Text] [PDF]

Home page
 CJASNHome page
S. Kato, M. Chmielewski, H. Honda, R. Pecoits-Filho, S. Matsuo, Y. Yuzawa, A. Tranaeus, P. Stenvinkel, and B. Lindholm
Aspects of Immune Dysfunction in End-stage Renal Disease
Clin. J. Am. Soc. Nephrol., September 1, 2008; 3(5): 1526 - 1533.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
N. H. R. Litjens, M. Huisman, M. van den Dorpel, and M. G. H. Betjes
Impaired Immune Responses and Antigen-Specific Memory CD4+ T Cells in Hemodialysis Patients
J. Am. Soc. Nephrol., August 1, 2008; 19(8): 1483 - 1490.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.