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NDT Advance Access published online on October 18, 2006
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfl497
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© The Author [2006]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received April 13, 2006
Accepted July 24, 2006

Original Article

Disproportionally low clearance of macromolecules from the plasma to the peritoneal cavity in a mouse model of peritoneal dialysis (PD)

Anna Rippe 1, Catarina Rippe 1, Karl Swärd 2, and Bengt Rippe 1 *

1 Department of Nephrology, University Hospital of Lund, Sweden
2 Department of Experimental Medical Science, BMC F12, Lund University, Sweden

* To whom correspondence should be addressed.
Bengt Rippe, E-mail: Bengt.Rippe{at}med.lu.se



  Abstract

Background. This study was performed to establish a model for quantitative measurements of a number of basic peritoneal transport parameters, particularly transperitoneal clearances (Cl) of macromolecules, during mouse peritoneal dialysis.

Methods. Mice were anaesthetized using 3% isofluorane inhalation anaesthesia. The right jugular vein and the left femoral artery were cannulated for infusion and sampling purposes and for registration of (mean) arterial blood pressure. Access to the peritoneal cavity occurred via a thin abdominal catheter (Ø 0.7 mm). About 2.5 ml of either 4% (n = 9) or 1.5% (n = 5) glucose containing PD-fluid were instilled intraperitoneally (i.p.). Dialysate volume was followed vs time using i.p. RISA (125I human serum albumin) as a volume marker, after correcting for RISA mass disappearance from the peritoneum, assessed separately (n = 11). Microsampling (10 µl) of plasma and dialysate was performed for determinations of glucose, haematocrit, radioactivity (RISA and 51Cr-EDTA) and Ficoll.

Results. The i.p. volume vs time curves [VD(t)] were, after scaling, similar to those observed in humans (and in rats). Clearance of RISA out of the peritoneal cavity (Clout) was 9.33 ± 0.83 µl/min and the clearance of RISA to plasma (Cl->P) and the RISA clearance to the peritoneal cavity (Cl->D) were 1.49 ± 0.13 and 0.084 ± 0.008 µl/min, respectively. The peritoneal transport coefficients for 51Cr-EDTA and glucose, as well as Clout and Cl->P, were 13-17% of those previously assessed in 300 g rats, whereas Cl->D was only ~2% of that in rat.

Conclusions. All peritoneal transport parameters measured, except Cl->D, scaled very well to the corresponding human data. The mechanisms of the disproportionally low clearance of macromolecules from the plasma to the peritoneal cavity in mice remain elusive and warrant further study.

Keywords: albumin; capillary permeability; Ficoll; lymph flow; peritoneal dialysis; pore modelling; transcapillary escape rate.
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