NDT Advance Access published online on August 25, 2006
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfl444
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Nephrology, lnstituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico
* To whom correspondence should be addressed. Background. Chronic renal damage is associated with inflammatory infiltration, fibrosis and vascular lesion, coupled with increased expression of cyclo-oxygenase 2 (COX-2) and transforming growth factor- Methods. Four groups of rats were studied: sham, 5/6 Nx, 5/6 Nx + aminoguanidine (AG) and 5/6 NX + L-NIL (L-N6-iminoethyl-lysine). Systolic blood pressure (SBP), proteinuria and creatinine (Cr) clearance were measured. NOS-2, COX-2 and TGF- Results. Systemic hypertension and marked proteinuria, increased expression of NOS-2, COX-2 and TGF- Conclusion. This study shows that NOS-2 was markedly enhanced in renal tissue of 5/6 Nx rats. Moreover, treatment with AG and L-NIL prevented the morpho-functional changes induced by subtotal renal ablation, despite persistence of systemic hypertension, suggesting that high concentrations of nitric oxide produced by NOS-2 could act as a positive modulator of the proinflammatory and profibrotic pathways involved in the progression of renal disease.
Received June 7, 2006
Accepted June 27, 2006
Original Article
Chronic inhibition of nos-2 ameliorates renal injury, as well as COX-2 and TFG-
Pablo Bautista-García 1, Laura Gabriela Sánchez-Lozada 1 *, Magdalena Cristóbal-García 1, Edilia Tapia 1, Virgilia Soto 2, Ma. Carmen Ávila-Casado 2, Ricardo Márquez-Velasco 3, Rafael Bojalil 3, Martha Franco 1, and Jaime Herrera-Acosta 1
1 overexpression in 5/6 nephrectomized rats
2 Department of Pathology, lnstituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico
3 Department of Immunology, lnstituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico
Laura Gabriela Sánchez-Lozada, E-mail: lgsanchezlozada{at}hotmail.com
Abstract 
1 (TGF-1). However, the role of inducible nitric oxide synthase (NOS-2) is still controversial. Thus, we studied the contribution of NOS-2 to the expression levels of COX-2 and TGF-1, as well as the structural renal injury in rats with subtotal renal ablation (5/6 Nx).1 gene expression was determined by real-time reverse transcription-polymerase-chain reaction. Protein expression was evaluated by western blot and ELISA (TGF-1). Immunohistochemistry and morphometry were performed for NOS-2, microvascular thickening and fibrosis.1, thickening of arteriolar wall and tubulointerstitial fibrosis were produced in 5/6 Nx rats. Chronic inhibition of NOS-2 did not prevent arterial hypertension or the fall in Cr clearance, but partially reduced proteinuria. Nevertheless, AG and L-NIL preserved arteriolar morphology and the administration of both selective inhibitors of inducible NOS (AG and L-NIL) prevented NOS-2 overexpression.1.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Tapia, D. J. Sanchez-Gonzalez, O. N. Medina-Campos, V. Soto, C. Avila-Casado, C. M. Martinez-Martinez, R. J. Johnson, B. Rodriguez-Iturbe, J. Pedraza-Chaverri, M. Franco, et al. Treatment with pyrrolidine dithiocarbamate improves proteinuria, oxidative stress, and glomerular hypertension in overload proteinuria Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1431 - F1439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cruz, R. Correa-Rotter, D. J. Sanchez-Gonzalez, R. Hernandez-Pando, P. D. Maldonado, C. M. Martinez-Martinez, O. N. Medina-Campos, E. Tapia, D. Aguilar, Y. I. Chirino, et al. Renoprotective and antihypertensive effects of S-allylcysteine in 5/6 nephrectomized rats Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1691 - F1698. [Abstract] [Full Text] [PDF]
|
|