Skip Navigation



NDT Advance Access published online on July 28, 2006
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfl434
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
21/11/3122    most recent
gfl434v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Caramori, M. L.
Right arrow Articles by Mauer, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Caramori, M. L.
Right arrow Articles by Mauer, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received December 8, 2005
Accepted June 22, 2006

Original Article

Cellular basis of diabetic nephropathy: IV Antioxidant enzyme mRNA expression levels in skin fibroblasts of type 1 diabetic sibling pairs

Maria Luiza Caramori 1, Youngki Kim 2, Paola Fioretto 3, Chunmei Huang 2, Stephen S. Rich 4, Michael E. Miller 4, Gregory B. Russell 4, and Michael Mauer 2 *

1 Department of Pediatrics, University of Minnesota, Minnesota, USA; Department of Medicine, University of Minnesota, Minnesota, USA
2 Department of Pediatrics, University of Minnesota, Minnesota, USA
3 Department of Medical and Surgical Sciences, University of Padova, Italy
4 Department of Public Health Sciences, Wake Forest School of Medicine, North Carolina, USA

* To whom correspondence should be addressed.
Michael Mauer, E-mail: mauer002{at}umn.edu



  Abstract

Background. Blunted cultured skin fibroblast (SF) antioxidant enzyme responses to hyperglycaemia are associated with diabetic nephropathy risk. The present study explores whether this association is, at least in part, genetically determined.

Methods. We measured glomerular structure and SF mRNA expression for catalase and glutathione peroxidase in 21 sibling pairs concordant for type 1 diabetes. All patients had four or more (mean 21.5) years of diabetes and glomerular filtration rate >40 ml/min/1.73 m2. Thirty-four patients were normoalbuminuric, four were microalbuminuric, three were proteinuric and one was not classifiable. Heritability of patient characteristics was assessed by intra-class correlation and by a genetic variance component model.

Results. Mesangial fractional volume, mesangial matrix fractional volume, glomerular basement membrane width and surface density of peripheral glomerular basement membrane per glomerulus were significantly correlated in these sibling pairs. Catalase mRNA expression levels were also related and highly heritable in these sibling pairs. The association between sibship and glutathione peroxidase mRNA expression levels did not reach statistical significance.

Conclusions. This study suggests that SF catalase mRNA expression levels, known to be associated with diabetic nephropathy risk, are in part genetically determined.

Keywords: genetics of diabetic nephropathy; glomerular structure; type 1 diabetes.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.