Skip Navigation



NDT Advance Access published online on October 4, 2005
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfi165
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
20/12/2636    most recent
gfi165v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Sugiura, H.
Right arrow Articles by Nihei, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sugiura, H.
Right arrow Articles by Nihei, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
Received December 20, 2004
Accepted August 30, 2005

Original Articles

Klotho reduces apoptosis in experimental ischaemic acute renal failure

Hidekazu Sugiura 1, Takumi Yoshida 2*, Ken Tsuchiya 1, Michihiro Mitobe 1, Sayoko Nishimura 1, Satsuki Shirota 1, Takashi Akiba 2, and Hiroshi Nihei 1

1 Department of Medicine IV, Tokyo Women's Medical University, Tokyo, Japan
2 Department of Medicine IV, Tokyo Women's Medical University, Tokyo, Japan; Department of Blood Purification, Tokyo Women's Medical University, Tokyo, Japan

* To whom correspondence should be addressed.
Takumi Yoshida, E-mail: tyoshida{at}kc.twmu.ac.jp



  Abstract

Background. Klotho is associated with the suppression of several ageing phenotypes. Because high klotho gene expression was detected in the kidney and several studies have found altered expression in animal models, we explored the physiological relevance of klotho expression in the kidney under renal ischemia reperfusion injury (IRI).

Methods. Male Wistar rats were subjected to bilateral renal ischemia or sham operation, followed by reperfusion for 6, 12 or 24 h, or 2 to 10 days. Renal expression of klotho was assessed by real-time PCR or Western blotting. Creatinine levels were determined. Immunohistochemical studies and TUNEL staining were performed. An adenovirus harbouring the mouse klotho gene (ad-kl) was intravenously administered to one group of rats before renal IRI.

Results. Renal klotho mRNA and protein expressions were significantly reduced in IRI rats the first day after ischemia. Pre-treatment with ad-kl resulted in a robust induction of klotho mRNA and protein in the liver but not in the kidney. Ad-kl gene transfer improved serum creatinine and the histological changes. Apoptosis induced by IRI was attenuated following ad-kl administration.

Conclusion. The data suggest klotho to be involved in the pathophysiology of IRI. Downregulation of renal klotho exacerbates ischaemic acute renal failure, and klotho gene induction has therapeutic potential in managing ischaemic renal damage.

Keywords: adenovirus; apoptosis; ischemia reperfusion; klotho.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
H. Sugiura, T. Yoshida, M. Mitobe, S. Yoshida, S. Shiohira, K. Nitta, and K. Tsuchiya
Klotho reduces apoptosis in experimental ischaemic acute kidney injury via HSP-70
Nephrol. Dial. Transplant., September 10, 2009; (2009) gfp451v1.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
M. Kuro-o
Klotho in chronic kidney disease--What's new?
Nephrol. Dial. Transplant., June 1, 2009; 24(6): 1705 - 1708.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Haruna, N. Kashihara, M. Satoh, N. Tomita, T. Namikoshi, T. Sasaki, T. Fujimori, P. Xie, and Y. S. Kanwar
Amelioration of progressive renal injury by genetic manipulation of Klotho gene
PNAS, February 13, 2007; 104(7): 2331 - 2336.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
S. Tsuruoka, K. Nishiki, T. Ioka, H. Ando, Y. Saito, M. Kurabayashi, R. Nagai, and A. Fujimura
Defect in parathyroid-hormone-induced luminal calcium absorption in connecting tubules of Klotho mice
Nephrol. Dial. Transplant., October 1, 2006; 21(10): 2762 - 2767.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
E. Lewin and K. Olgaard
Klotho, an important new factor for the activity of Ca2+ channels, connecting calcium homeostasis, ageing and uraemia
Nephrol. Dial. Transplant., July 1, 2006; 21(7): 1770 - 1772.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.