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NDT Advance Access published online on April 6, 2005
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfh764
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© The Author [2005]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received September 29, 2004
Accepted February 2, 2005

Original Articles

Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes

Chia-Chao Wu 1, Jin-Shuen Chen 2, Wen-Mein Wu 3, Tung-Nan Liao 4, Pauling Chu 2, Shih-Hua Lin 2, Chien-Huei Chuang 2, and Yuh-Feng Lin 2*

1 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei; Division of Nephrology, Department of Internal Medicine, Tri-Service General Hospital, Taipei
2 Division of Nephrology, Department of Internal Medicine, Tri-Service General Hospital, Taipei
3 Department of Nutrition and Food Sciences, Fu Jen Catholic University, Taipei
4 Department of Medical Technology, Chung-Hwa College of Medical Technology, Tainan, Taiwan

* To whom correspondence should be addressed.
Yuh-Feng Lin, E-mail: linyf{at}ndmctsgh.edu.tw



  Abstract

Background. There is increased oxidative stress in patients undergoing haemodialysis (HD); however, little is known of how different dialysis membranes contribute to the oxidative stress induced by the dialysis procedure per se. We therefore studied the influence of two different dialysis membranes on oxidative stress during HD.

Methods. Eight patients undergoing HD three times per week were enrolled in this cross-controlled study. Patients sequentially received HD using polysulphone (PS) and regenerated cellulose (RC) dialysis membranes for 1 week each. Blood samples were collected in the last section of each hollow fibre 0, 15, 120 and 240 min after starting HD. We determined superoxide anion production derived from neutrophils, superoxide dismutase (SOD) and glutathione peroxidase (GPx) derived from washed red cells, plasma myeloperoxidase (MPO), plasma thiobarbituric acid-reactive substances (TBARS), plasma advanced oxidation protein products (AOPP) and serum 8-hydroxy-2'-deoxyguanosine (8-OHdG).

Results. Leukocyte numbers, including neutrophils, lymphocytes and monocytes, decreased significantly after 15 min of dialysis, more so with RC than with PS membrane. For both membranes, superoxide anion production transiently increased during the first 15 min whereas the post-dialysis production was decreased. Plasma MPO levels persistently increased during dialysis with the two membranes. Moreover, the increase was more marked with RC than with PS membrane. AOPP and 8-OHdG levels increased progressively when using RC membranes. There were no significant differences in SOD, GPx, TBARS, AOPP and 8-OHdG levels between the two membranes.

Conclusions. The biocompatibility of the dialyser affects oxidative stress production during a single dialysis session. The measurement of MPO may serve as a reliable marker of the degree of oxidative stress induced using dialysis membranes of different biocompatibilities.

Keywords: antioxidants; biocompatibility; haemodialysis; myeloperoxidase; oxidative stress.
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