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NDT Advance Access published online on March 15, 2005
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfh758
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© The Author [2005]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received May 11, 2004
Accepted January 5, 2005

Brief Report

Effect of L-carnitine on erythroid colony formation in mouse bone marrow cells

Yukika Kitamura 1*, Kazunori Satoh 2, Takashi Satoh 3, Manabu Takita 1, and Akihiro Matsuura 1

1 Nephrology and Dialysis Research Drug Discovery Department II, Kawasaki, Japan
2 Pharmacology of Parenteral & Enteral Nutrition and Dialysis (PEN&D), PEN&D Research Laboratories, Pharmaceutical Research Laboratories, Kawasaki, Japan
3 Clinical Development Department, Ajinomoto Co., Inc.,Kawasaki, Japan

* To whom correspondence should be addressed.
Yukika Kitamura, E-mail: yukika_kitamura{at}ajinomoto.com



  Abstract

Background. L-Carnitine can alleviate uraemic anaemia in haemodialysis patients by improving erythrocyte membrane functions or erythropoiesis, which are depressed under uraemic conditions. L-Carnitine and palmitoyl-L-carnitine were reported to increase the formation of colony-forming unit-erythroid (CFU-E) colonies in cultures of fetal mouse liver cells, an effect that depended on the concentration of palmitoyl-L-carnitine but not of L-carnitine. In this study, we investigated L-carnitine's effect on CFU-E colony formation in cell cultures of mouse bone marrow cells.

Methods. Bone marrow from normal female mice was placed in 35 mm culture dishes containing a medium composed of methylcellulose and various nutrients. The dishes were incubated for 48 h, and the colonies of erythroblasts, which were differentiated from CFU-E, consisting of ≥8 cells, were counted in each dish using an inverted microscope.

Results. The numbers of CFU-E colonies correlated well with both the initial numbers of bone marrow cells and concentrations of recombinant human erythropoietin (rhEPO) in the methylcellulose medium. In the presence of 0.5 or 1.0 IU/ml of rhEPO, L-carnitine at concentrations of 200 and 400 µmol/l significantly enhanced CFU-E colony formation (P<0.001).

Conclusion. L-Carnitine significantly increased the number of CFU-E colonies in mouse bone marrow cell cultures. This finding suggests that L-carnitine stimulates erythropoiesis, partially accounting for its mitigating effect on renal anaemia.

Keywords: anaemia; L-carnitine; CFU-E; erythropoiesis; mouse bone marrow cell; rhEPO.
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