Skip Navigation



NDT Advance Access published online on July 20, 2004
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfh399
© 2004 by European Renal Association - European Dialysis and Transplant Association
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
19/9/2198    most recent
gfh399v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ozgen, N.
Right arrow Articles by Saito, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ozgen, N.
Right arrow Articles by Saito, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received December 25, 2003
Accepted March 24, 2004

Original Article

Evaluation of long-term transport ability of a bioartificial renal tubule device using LLC-PK1 cells

Nazira Ozgen 1, Masuo Terashima 1, Tun Aung 1, Yashinobu Sato 2, Chie Isoe 1, Takatoshi Kakuta 2, Akira Saito 1*

1 Department of Molecular and Cellular Nephrology, Institute of Medical Sciences, Tokai University, Kanagawa, Japan
2 Division of Nephrology, Tokai University School of Medicine, Kanagawa, Japan

* To whom correspondence should be addressed. E-mail: asait{at}is.icc.u-tokai.ac.jp.



  Abstract

Background. Haemodialysis therapy does not provide renal tubule function, such as active fluid and solute transport, nor metabolic or endocrine action. Moreover, this treatment is usually associated with serious complications and high mortality. We constructed a bioartificial renal tubule device by using renal tubule epithelial cells in an artificial membrane, and evaluated transport properties of the device for 2 weeks.

Methods. A renal epithelial cell line, LLC-PK1 (Lewis-lung cancer porcine kidney), was seeded on polysulfone hollow fibres in small and large modules. We studied perfusion and leakage of urea nitrogen (UN) and creatinine (Cr), as well as reabsorption of water, glucose and sodium for a period of 2 weeks.

Results. Cell-lined hollow fibre membranes significantly reduced the leakage of UN and Cr throughout the 2 week period. Reabsorption of water, glucose and sodium were adequate from days 3 to 10 and gradually decreased thereafter. LLC-PK1 cells actively transported these substances. Scanning electron microscopy revealed that cells in the hollow fibres on day 8 became completely confluent. However, they became multi-layered and almost obstructed the hollow fibres on day 13.

Conclusions. This bioartificial renal tubule device functioned to reabsorb water, glucose and sodium for ~10 days. This is the first report of successful long-term evaluation of a bioartificial renal tubule device. This device, in combination with continuous haemofiltration, may provide treatment to prevent complications of dialysis and raise the quality of life in chronic renal failure patients.

Keywords: bioartificial renal tubule device; chronic renal failure; continuous haemofiltration; LLC-PK1 cells.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.