Skip Navigation


NDT Advance Access originally published online on December 4, 2008
Nephrology Dialysis Transplantation 2009 24(2):661-666; doi:10.1093/ndt/gfn656
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
24/2/661    most recent
gfn656v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Wiggins, K. J.
Right arrow Articles by Langham, R. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wiggins, K. J.
Right arrow Articles by Langham, R. G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2008]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org


BK virus RNA can be detected in archival renal transplant biopsies using the reverse trancription polymerase chain reaction

Kathryn J. Wiggins1,2, Renae M. Gow1, John Kanellis3, Prue Hill4, Darren J. Kelly1, Alison Skene5, David J. Goodman2 and Robyn G. Langham1,2

1 Department of Medicine, St Vincent's Hospital, University of Melbourne, Clinical Sciences Building, Cnr Princes and Regent Streets, Fitzroy Victoria 3065 2 Department of Nephrology, St. Vincent's Hospital, PO Box 2900, Fitzroy Victoria 3065 3 Department of Nephrology, Monash Medical Centre, 246 Clayton Road, Clayton Victoria 3168 4 Department of Anatomical Pathology, St. Vincent's Hospital, PO Box 2900, Fitzroy, Victoria 3065 5 Department of Anatomical Pathology, Monash Medical Centre. 246 Clayton Road, Clayton Victoria 3168, Australia

Correspondence and offprint requests to: Kathryn J. Wiggins, Department of Medicine, St. Vincent's Hospital, University of Melbourne, Level 4, Clinical Sciences Building, Cnr Princes and Regent Streets, Fitzroy VIC 3065, Australia. Tel: +61-3-9288-2211; Fax: +61-3-9288-3151; E-mail: kwiggins@medstv.unimelb.edu.au

Keywords: archival renal biopsy; BK virus; PCR; renal transplant; RNA

The first 150 words of the full text of this article appear below.



   Introduction
 
BK virus (BKV) nephropathy (BKVN), also known as polyoma virus nephropathy (PVAN), is a significant cause of allograft dysfunction and loss in renal transplant recipients. Early reports of this condition suggested that the rate of graft failure due to BKVN was 50–80% [1–3]. Since the publication of these reports, increased awareness and progressively widespread availability of PCR assays to detect viral DNA in urine and blood have led to early recognition and diagnosis of this condition [4]. However, a contemporary case series of patients who received transplants in a centre where PCR was used as a screening tool still reported major decline in graft function in 24% of affected individuals, and graft loss in 15% [5]. Initial treatment for BKVN involves reduction of immunosuppression. Although generally well tolerated, this is associated with a risk of subsequent acute rejection (AR) [6,7]. These findings . . . [Full Text of this Article]



   Materials and methods
 
Subjects
Renal biopsies
RNA extraction
Polymerase chain reaction (PCR)
Histological analysis
Quantification of tissue fibrosis
Statistical analysis


   Results
 
Patient characteristics
BKVN in renal biopsies
BKV RNA detection
Relationship between relative viral load and clinical course
Comparison of RT-PCR and histology


   Discussion
 


   Supplementary data
 

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?