Skip Navigation


NDT Advance Access originally published online on December 22, 2007
Nephrology Dialysis Transplantation 2008 23(6):2016-2023; doi:10.1093/ndt/gfm899
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
23/6/2016    most recent
gfm899v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gerrits, J. H.
Right arrow Articles by Besouw, N. M. v.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gerrits, J. H.
Right arrow Articles by Besouw, N. M. v.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2007]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org


Donor-reactive cytokine profiles after HLA-identical living-related kidney transplantation

Jeroen H. Gerrits1, Jacqueline van de Wetering1, Jos J.M. Drabbels2, Frans H.J. Claas2, Willem Weimar1 and Nicole M. van Besouw1

1 Department of Internal Medicine – Transplantation, Erasmus MC, University Medical Center Rotterdam, Rotterdam 2 Department of Immunohaematology and Blood transfusion, Leiden University Medical Center, Leiden, The Netherlands

Correspondence and offprint requests to: Jeroen H. Gerrits, Department of Internal Medicine – Transplantation, Room Ee-563a, Erasmus MC, University Medical Center Rotterdam, PO Box 2040, NL-3000 CA, Rotterdam, The Netherlands. Tel: +31-10-703-4521; Fax: +31-10-704-4718; E-mail: j.gerrits{at}erasmusmc.nl



  Abstract

Background. After HLA-identical living-related (LR) kidney transplantation, only non-HLA antigen mismatches between donor and recipient may exist. We questioned whether donor-reactive responses against non-HLA antigens could be found after HLA-identical LR kidney transplantation, and wondered whether donor reactivity in the HLA-identical setting was different from the HLA-mismatched setting during immunological quiescence. Healthy individuals served as controls.

Methods. Elispot assays were performed to determine the number of alloreactive IFN-{gamma}-producing cells (pc), IL-10 pc, granzyme B (GrB) pc and IL-13 pc from peripheral blood mononuclear cells (PBMC) of HLA-identical, HLA-mismatched LR kidney transplant recipients and healthy individuals.

Results. The frequency of alloreactive IFN-{gamma} pc, IL-13 pc and GrB pc was higher in healthy individuals compared to both transplant patient groups. In the HLA-identical group, significantly higher numbers of donor-reactive IL-10 pc were found compared to their autologous control. These frequencies were also higher compared to the HLA-mismatched and healthy control group. The number of donor-reactive GrB pc was higher in the HLA-mismatched group than in the HLA-identical group. Donor-reactive IFN-{gamma} pc and IL-13 pc were comparable in both transplant groups.

Conclusions. In recipients of HLA-identical LR kidney transplant, high donor-reactive IL-10 pc, in combination with low donor-reactive IFN-{gamma} pc, IL-13 pc and GrB pc, suggests active downregulation of reactivity against non-HLA molecules.

Keywords: alloreactivity; Elispot assay; IFN-{gamma}; IL-10; kidney transplantation; minor histocompatibility antigens; non-HLA antigens

Received for publication: 10. 8.07
Accepted in revised form: 26.11.07


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.