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NDT Advance Access originally published online on May 25, 2007
Nephrology Dialysis Transplantation 2007 22(10):2886-2893; doi:10.1093/ndt/gfm301
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© The Author [2007]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org


Adult and paediatric patients with minimal change nephrotic syndrome show no major alterations in glomerular expression of sulphated heparan sulphate domains

Tessa J. M. Wijnhoven1,3, Joyce M. Geelen3, Marinka Bakker2, Joost F. M. Lensen1, Angelique L. W. M. M. Rops2, Andrea B. Kramer5, Gerjan Navis5, Mabel J. W. van den Hoven2, Johan van der Vlag2, Jo H. M. Berden2,4, Jack F. M. Wetzels4, Lambert P. W. J. van den Heuvel3, Leo A. H. Monnens3 and Toin H. van Kuppevelt1

1Department of Matrix Biochemistry, 2Nephrology Research Laboratory, Nijmegen Centre for Molecular Life Sciences, 3Department of Pediatric Nephrology, 4Division of Nephrology, Radboud University Nijmegen Medical Centre, Nijmegen, and 5Department of Pathology, University Medical Center Groningen, Groningen, The Netherlands.

Correspondence and offprint requests to: Toin H. van Kuppevelt, Department of Matrix Biochemistry, Nijmegen Centre for Molecular Life Sciences, Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. Email: A.vankuppevelt{at}ncmls.ru.nl



  Abstract

Background. Minimal change nephrotic syndrome (MCNS) is the most frequent form of nephrotic syndrome in childhood. In the glomerular basement membrane (GBM) of adult patients with MCNS, a reduced expression of a specific heparan sulphate (HS) domain has been reported. In children with MCNS, urinary activity of the HS-degrading enzyme heparanase was increased. It is, therefore, possible that a decreased GBM HS expression is associated with the pathogenesis of proteinuria in patients with MCNS.

Methods. In this study, HS in glomeruli of five adult and six paediatric patients with MCNS were analysed by immunofluorescence staining using four different antibodies, each defining a specific sulphated HS domain. The pediatric patients were subdivided into three groups depending on the presence or absence of podocyte foot process effacement, the level of proteinuria and prednisone administration at the time of the biopsy. In addition, kidneys of rats with adriamycin nephropathy (ADRN), a model for MCNS, were included in the study.

Results. Expression of sulphated HS domains was not aberrant in adult or paediatric patients compared with control subjects. Children with and without proteinuria had the same HS content. In contrast, rats with ADRN showed a decreased glomerular expression of sulphated HS domains.

Conclusions. These results suggest that in patients with MCNS proteinuria is not associated with major changes in glomerular expression of sulphated HS domains.

Keywords: heparan sulphate; immunofluorescence staining; kidney; minimal change nephrotic syndrome; proteinuria, sulphation

Received for publication: 22. 3.07
Accepted in revised form: 20. 4.07


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