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NDT Advance Access originally published online on February 8, 2005
Nephrology Dialysis Transplantation 2005 20(4):768-774; doi:10.1093/ndt/gfh697
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© The Author [2005]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Original Article

Influence of standard haemodialysis treatment on transcription of human serum- and glucocorticoid-inducible kinase SGK1 and taurine transporter TAUT in blood leukocytes

Björn Friedrich1, Dorothea Alexander2, Wilhelm Karl Aicher2, Michael Duszenko3, Thomas Patrick Schaub5, Jutta Passlick-Deetjen5, Siegfried Waldegger6, Sabine Wolf1, Teut Risler1 and Florian Lang4

1 Department of Internal Medicine, 2 Department of Orthopedics, 3 Department of Biochemistry and 4 Department of Physiology, University of Tübingen, 5 Fresenius Medical Care, Bad Homburg, and 6 Department of Paediatrics, University of Marburg, Germany

Correspondence and offprint requests to: Prof. med. Florian Lang, Physiologisches Institut I, Gmelinstrasse 5, 72076 Tübingen, Germany. Email: florian.lang{at}uni-tuebingen.de

Background. Standard haemodialysis (HD) rapidly alters osmolality and composition of extracellular fluid and, thus, challenges cell volume constancy. Cell volume-sensitive genes upregulated by osmotic cell shrinkage include those encoding for taurine transporter TAUT as well as for serum- and glucocorticoid-inducible kinase SGK1.

Methods. Six HD patients were haemodialysed for 4 h with high-flux dialysers. Blood was drawn from the arterial section of the fistula immediately prior to start of HD and subsequently after 60, 120 and 240 min of HD treatment and, in addition, 120 min after HD treatment. Taurine plasma concentrations ([taurine]p) and erythrocytic taurine content ([taurine]e) were determined by high-performance liquid chromatography. SGK1 and TAUT transcript levels in leukocytes were quantified by real-time polymerase chain reaction.

Results. The [taurine]p was significantly higher in HD patients before HD treatment when compared with healthy controls and it decreased significantly during 4 h of HD. The ratio of SGK1/GAPDH and of TAUT/GAPDH transcript levels increased significantly by 50% or 27%, respectively, during HD.

Conclusions. Standard HD treatment decreases plasma taurine concentration and upregulates leukocyte SGK1 and TAUT transcription. As SGK1 is a potent regulator of ion channels and transporters in nervous system, heart muscle and epithelial cells, the deranged regulation of SGK1 may contribute to acute side effects of HD treatment.

Keywords: cell volume regulation; osmolytes; real-time polymerase chain reaction; SGK1; taurine transporter


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