NDT Advance Access originally published online on June 14, 2005
Nephrology Dialysis Transplantation 2005 20(10):2060-2070; doi:10.1093/ndt/gfh939
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Renal accumulation of pentosidine in non-diabetic proteinuria-induced renal damage in rats
1 Department of Nephrology, and 2 Department of Pathology and Laboratory Medicine, University Medical Center Groningen, University of Groningen, The Netherlands, 3 Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, USA, 4 Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto and 5 Department of Pathology, Fukuoka University, School of Medicine, Fukuoka, Japan
Correspondence and offprint requests to: G. J. Navis, University Medical Center Groningen, Department of Nephrology, PO Box 30.001, 9700 RB Groningen, The Netherlands. Email: G.J.Navis{at}int.umcg.nl
Background. Advanced glycation end-products (AGEs) contribute to the pathogenesis of diabetic glomerulopathy. The role of AGEs in non-diabetic renal damage is not well characterized. First, we studied whether renal AGE accumulation occurs in non-diabetic proteinuria-induced renal damage and whether this is ameliorated by renoprotective treatment. Secondly, we investigated whether renal AGE accumulation was due to intrarenal effects of local protein trafficking.
Methods. Pentosidine was measured (by high-performance liquid chromatography) in rats with chronic bilateral adriamycin nephropathy (AN), untreated and treated with lisinopril. Age-matched healthy rats served as negative controls. Secondly, we compared renal pentosidine in mild proteinuric and non-proteinuric kidneys of unilateral AN and in age-matched controls at 12 and 30 weeks. Intrarenal localization of pentosidine was studied by immunohistochemistry.
Results. Renal pentosidine was elevated in untreated AN (0.14±0.04 µmol/mol valine) vs healthy controls (0.04±0.01 µmol/mol valine, P<0.01). In lisinopril-treated AN, pentosidine was lower (0.09±0.02 µmol/mol valine) than in untreated AN (P<0.05). In unilateral proteinuria, pentosidine was similar in non-proteinuric and proteinuric kidneys. After 30 weeks of unilateral proteinuria, pentosidine was increased in both kidneys (0.26±0.10 µmol/mol valine) compared with controls (0.18±0.06 µmol/mol valine, P<0.05). Pentosidine (AN, week 30) was also increased compared with AN at week 12 (0.16±0.06 µmol/mol valine, P<0.01). In control and diseased kidneys, pentosidine was present in the collecting ducts. In proteinuric kidneys, in addition, pentosidine was present in the brush border and cytoplasm of dilated tubular structures, i.e. at sites of proteinuria-induced tubular damage.
Conclusion. Pentosidine accumulates in non-diabetic proteinuric kidneys in damaged tubules, and renoprotective treatment by angiotensin-converting enzyme (ACE) inhibitors inhibits AGE accumulation, supporting a relationship between abnormal renal protein trafficking, proteinuria-induced tubular damage and tubular pentosidine accumulation. Future studies, applying specific AGE inhibitors, should be conducted to provide insight into the pathophysiological significance of renal AGEs in non-diabetic renal disease.
Keywords: Advanced glycation end products; Maillard reaction; Nonenzymatic glycosylation; adriamycin nephrosis; angiotensin converting enzyme (ACE) inhibition; immunohistochemistry
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