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NDT Advance Access originally published online on February 19, 2004
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Nephrol Dial Transplant (2004) 19: 1298-1301
Nephrol Dial Transplant Vol. 19 No. 5 © ERA-EDTA 2004; all rights reserved

Technical Note

Genotyping: a new application for the spent dialysate in peritoneal dialysis

Gaëlle Gillerot, Huguette Debaix and Olivier Devuyst

Division of Nephrology, Université Catholique de Louvain Medical School, Brussels, Belgium

Correspondence and offprint requests to: Olivier Devuyst, Division of Nephrology, UCL Medical School, 10 Avenue Hippocrate, B-1200 Brussels, Belgium. Email: devuyst{at}nefr.ucl.ac.be

Abstract

Background. The dialysate of patients on peritoneal dialysis (PD) is used to determine the concentration of growth factors and cytokines, and as a source of resident peritoneal cells for subsequent culture experiments. We hypothesized that the cells contained in spent dialysate samples obtained at the time of the peritoneal equilibration test (PET) and subsequently stored may represent a source of DNA from a given PD patient.

Methods. We characterized a protocol of DNA extraction from dialysates obtained in PD patients after a long dwell during the initial PET and kept frozen up to several years. After amplification of the source DNA by strand displacement using the bacteriophage {phi}29 DNA polymerase, we performed polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis for the Glu298Asp polymorphism of ENOS to demonstrate the suitability of the extracted DNA for genotyping.

Results. A significant amount of DNA (mean yield 12 µg/ml dialysate) was extracted from frozen dialysate samples. The extraction yield was not influenced by the duration of storage at –20°C. Following amplification, the DNA extracted from the dialysate was used successfully for genotyping the Glu298Asp polymorphism of ENOS, as demonstrated by parallel analyses using DNA extracted from the peripheral blood and sequencing.

Conclusions. These results demonstrate that the dialysis effluent obtained at the time of the initial PET and stored at –20°C is a reliable source of DNA that can be used subsequently for PCR amplification, RFLP analysis and sequencing.

Keywords: DNA; peritoneal dialysis; PET; PCR; polymorphism


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