Nephrol Dial Transplant (2004) 19: 158-163
© ERA–EDTA 2003; all rights reserved
Original Article
Increased hydrogen peroxide in the exhaled breath of uraemic patients unaffected by haemodialysis
nicki31Department of Internal Medicine and Dialysotherapy Medical University of Lodz, Lodz, 2Practical Clinical Training Centre, Medical University of Lodz, Lodz and 3Department of Experimental and Clinical Physiology, Institute of Physiology and Biochemistry, Medical University of Lodz, Lodz, Poland
Correspondence and offprint requests to: Dariusz Nowak, Department of Experimental and Clinical Physiology, Institute of Physiology and Biochemistry, Medical University of Lodz, Mazowiecka Str. 6/8, 92-215 Lodz, Poland. Email: dnowak{at}zdn.am.lodz.pl
Background. Uraemia is accompanied by conditions favouring the rise of H2O2 activity in body fluids. This results from the increased release of H2O2 by polymorphonuclear leukocytes and decreased plasma glutathione peroxidase activity. The purpose of this study was to determine if patients on chronic haemodialysis (HD) exhale more H2O2 than healthy individuals, and if dialysis affects breath H2O2 content.
Methods. We studied 29 chronic HD patients (mean age 49 ± 11 years) and 40 healthy persons (mean age 44 ± 9 years). H2O2, which is volatile, was measured fluorimetrically with the homovanillic acid method in the exhaled breath condensate (EBC) of the study cohort. EBC was collected immediately before and after the HD session and also at 20 and 60 min of HD treatment (n = 14) and once in controls. Peak expiratory flow (PEF), white blood cell (WBC) count, PaO2 and circulatory cyclic guanosine monophosphate (cGMP), Il-6 and Il-8 concentrations were measured concomitantly. Finally, H2O2 diffusion through the dialyser cuprophane membrane was determined in an in vitro experiment.
Results. At baseline, EBC H2O2 concentration was 22 times higher in HD patients than in controls (2.92 ± 4.64 vs 0.16 ± 0.13 µM, P < 0.001). Although the maximum decrease in PEF (431 ± 52 vs 398 ± 56 l/min, P < 0.01) and WBC count (6.72 ± 1.02 vs 3.82 ± 1.51 x 103/µl, P < 0.01) occurred at 20 min after the start of HD, no significant changes in breath H2O2 levels were noted throughout the session. Plasma IL-6 and IL-8 levels remained unchanged whereas cGMP rose 1.3 times at 60 min (P < 0.01). In vitro, H2O2 rapidly diffused through the cuprophane membrane.
Conclusion. Chronic HD patients exhale more H2O2 than healthy subjects. Although no change of breath H2O2 concentration was observed during HD, as H2O2 easily diffuses through the dialyser membrane, it is not possible to rule out that HD stimulates H2O2 generation.
Keywords: Exhaled breath condensate; haemodialysis; hydrogen peroxide; uraemia
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  I. Horvath, J. Hunt, P. J. Barnes, and On behalf of the ATS/ERS Task Force on Exhaled Bre Exhaled breath condensate: methodological recommendations and unresolved questions Eur. Respir. J., September 1, 2005; 26(3): 523 - 548. [Abstract] [Full Text] [PDF]
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