Nephrol Dial Transplant (2001) 16: 1222-1229
© 2001 European Renal Association-European Dialysis and Transplant Association
Intra- and post-dialytic platelet activation and PDGF-AB release: cellulose diacetate vs polysulfone membranes
1 Department of Clinical Medicine and Applied Biotechnology, 2 Central Laboratory, 3 Institute of Hematology and Medical Oncology ‘L. & A. Seràgnoli’, St Orsola University Hospital, Bologna, Italy
Background. During haemodialysis the blood-membrane contact causes a release of platelet granule content, which contains platelet-derived growth factor AB (PDGF-AB). In view of the potential role of this in altering biocompatibility during haemodialysis, we evaluated the intra- and post-dialytic changes in PDGF-AB serum levels during haemodialysis sessions performed with cellulose diacetate (CDA) and polysulfone (PS) membranes respectively.
Methods. PDGF-AB, platelet factor 4 (PF4), beta thromboglobulin (ßTG), and mean platelet volume (MPV) levels were determined in 30 patients, each of whom underwent six dialysis sessions: three with a CDA and three with a PS membrane. Blood samples were taken at times 0, 15, 30, 120, 180, and 240 min during dialysis and at 1, 4, and 20 h after the end of the session. Statistical analysis was performed using a one-way ANOVA and Student's t test.
Results. PDGF-AB at 15 min was increased to +41±9% with CDA vs +20±5% with PS (P<0.001) from the T0 values, and at 120 min it was +19±8% with CDA vs -25±9% with PS (P<0.001) from T0 levels. At 240 min it was +95±14% with CDA vs +49±15% with PS (P<0.001) from the T0 values, returning to basal only 20 h after the end of the session. ßTG at 15 min was +60±8% for CDA vs +24±7.5% for PS (P<0.001) from the T0 values. PF4 showed a similar trend to ßTG. MPV at 30 min from the start of dialysis was 7.4±0.3 fl with CDA and 8±0.3 fl with PS (P<0.001), and at 240 min MPV was 7.9±0.3 fl with CDA and 8.4±0.3 fl with PS (P<0.001).
Conclusions. Platelet activation and platelet release reactions are lower with PS than with CDA membranes. PDGF-AB, released during and after dialysis, represents a clear biocompatibility marker. Its slow return to basal values and its action on vascular cells make it a potential risk factor for atherosclerosis in uraemic patients.
Keywords: biocompatibility; cellulose diacetate; haemodialysis; PDGF-AB release; platelet activation; polysulfone
Correspondence and offprint requests to: Dr Giuseppe Cianciolo, Department of Clinical Medicine and Applied Biotechnology, St Orsola University Hospital, via Massarenti 9, 40138 Bologna, Italy.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Gritters, P. Borgdorff, M. P. C. Grooteman, M. Schoorl, M. Schoorl, P. C. M. Bartels, G.-J. Tangelder, and M. J. Nube Platelet activation in clinical haemodialysis: LMWH as a major contributor to bio-incompatibility? Nephrol. Dial. Transplant., September 1, 2008; 23(9): 2911 - 2917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Wang, W. Beck, R. Deppisch, S. M. Marshall, N. A. Hoenich, and M. G. Thompson Differential effects of dialysis and ultrafiltrate from individuals with CKD, with or without diabetes, on platelet phosphatidylserine externalization Am J Physiol Renal Physiol, January 1, 2008; 294(1): F220 - F228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Richtrova, K. Opatrny Jr, L. Vit, F. Sefrna, and R. Perlik The AN69 ST haemodialysis membrane under conditions of two different extracorporeal circuit rinse protocols a comparison of thrombogenicity parameters Nephrol. Dial. Transplant., October 1, 2007; 22(10): 2978 - 2984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Naumnik, J. Borawski, K. Pawlak, and M. Mysliwiec Enoxaparin but not unfractionated heparin causes a dose-dependent increase in plasma TGF-{beta}1 during haemodialysis: a cross-over study Nephrol. Dial. Transplant., June 1, 2007; 22(6): 1690 - 1696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gritters, M. P. C. Grooteman, M. Schoorl, M. Schoorl, P. C. M. Bartels, P. G. Scheffer, T. Teerlink, C. G. Schalkwijk, M. Spreeuwenberg, and M. J. Nube Citrate anticoagulation abolishes degranulation of polymorphonuclear cells and platelets and reduces oxidative stress during haemodialysis Nephrol. Dial. Transplant., January 1, 2006; 21(1): 153 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Uehlinger, S. M. Jakob, P. Ferrari, M. Eichelberger, U. Huynh-Do, H.-P. Marti, M. G. Mohaupt, B. Vogt, H. U. Rothen, B. Regli, et al. Comparison of continuous and intermittent renal replacement therapy for acute renal failure Nephrol. Dial. Transplant., August 1, 2005; 20(8): 1630 - 1637. [Abstract] [Full Text] [PDF]
|
|