Nephrol Dial Transplant (2000) 15: 872-876
© 2000 European Renal Association-European Dialysis and Transplant Association
Brief Reports
Platelet-derived growth factor, basic fibroblast growth factor, and interferon 
increase type IV collagen production in human fetal mesangial cells via a transforming growth factor-ß-dependent mechanism
Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan
Background. Glomerulosclerosis is characterized by glomerular accumulation of extracellular matrix following mesangial cell proliferation. The precise pathomechanism of glomerulosclerosis is still undetermined. Platelet-derived growth factor (PDGF) and basic fibroblast growth factor (b-FGF) are known to be mitogenic for mesangial cells, and interferon 
(IFN-) is known to have an inhibitory effect on mesangial cell proliferation. We attempted to clarify the role of these cytokines on mesangial matrix production using cultured human fetal mesangial cells (HMC).
Methods. HMC were incubated with these cytokines for 24–72 h and the levels of type IV collagen and TGF-ß in the cell supernatants were measured by enzyme immunoassay.
Results. PDGF, b-FGF, and IFN- stimulated type IV collagen production by HMC in a dose- and time-dependent manner. The anti-TGF-ß neutralizing antibody clearly inhibited their stimulatory effect on type IV collagen production. PDGF and b-FGF also stimulated TGF-ß production by HMC in a dose-dependent manner, although IFN- did not.
Conclusion. PDGF, b-FGF, and IFN- stimulate type IV collagen production in cultured HMC via a TGF-ß-dependent mechanism.
Keywords: b-FGF; IFN-; mesangial cell; PDGF; TGF-ß; type IV collagen
Correspondence and offprint requests to:Hideaki Yamabe MD, Second Department of Internal Medicine, Hirosaki University School of Medicine, Zaifu-cho 5, Hirosaki, 036-8216 Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P. Vieitez, O. Gomez, E. R Uceda, M. E. Vera, and E. Molina-Holgado Systemic and local effects of angiotensin II blockade in experimental diabetic nephropathy Journal of Renin-Angiotensin-Aldosterone System, June 1, 2008; 9(2): 96 - 102. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Floege, F. Eitner, and C. E. Alpers A New Look at Platelet-Derived Growth Factor in Renal Disease J. Am. Soc. Nephrol., January 1, 2008; 19(1): 12 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Whitsett, C. J. Bachurski, K. C. Barnes, P. A. Bunn Jr., L. M. Case, D. N. Cook, D. Crooks, M. W. Duncan, L. Dwyer-Nield, R. C. Elston, et al. Functional Genomics of Lung Disease Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2/S1): S1 - S81. [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mitchell, K. Rodgers, J. Hanly, B. McMahon, H. R. Brady, F. Martin, and C. Godson Lipoxins Inhibit Akt/PKB Activation and Cell Cycle Progression in Human Mesangial Cells Am. J. Pathol., March 1, 2004; 164(3): 937 - 946. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Chen, Y. Wei, G. C. Sharp, and H. Braley-Mullen Inhibition of TGF{beta}1 by Anti-TGF{beta}1 Antibody or Lisinopril Reduces Thyroid Fibrosis in Granulomatous Experimental Autoimmune Thyroiditis J. Immunol., December 1, 2002; 169(11): 6530 - 6538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ostendorf, U. Kunter, C. van Roeyen, S. Dooley, N. Janjic, J. Ruckman, F. Eitner, and J. Floege The Effects of Platelet-Derived Growth Factor Antagonism in Experimental Glomerulonephritis Are Independent of the Transforming Growth Factor-{beta} System J. Am. Soc. Nephrol., March 1, 2002; 13(3): 658 - 667. [Abstract] [Full Text] [PDF]
|
|