Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Nishikawa, Y.
Right arrow Articles by Kohno, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nishikawa, Y.
Right arrow Articles by Kohno, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nephrol Dial Transplant (2000) 15: 772-777
© 2000 European Renal Association-European Dialysis and Transplant Association

Streptococcal M protein enhances TGF-ß production and increases surface IgA-positive B cells in vitro in IgA nephropathy

Yasuhiko Nishikawa, Ryujiro Shibata, Yoshiyuki Ozono, Hiroshi Ichinose, Masanobu Miyazaki, Takashi Harada and Shigeru Kohno

The Second Department of Internal Medicine, Nagasaki University School of Medicine, Nagasaki, Japan

Background. High serum levels and enhanced in vitro production of IgA are observed in more than half of patients with IgA nephropathy (IgAN); and transforming forming growth factor-ß (TGF-ß) is certain IgA class switching factor. On the other hand, macroscopic haematuria appears frequently with upper respiratory infection as tonsillitis in IgAN.

Methods. We compared the lymphocytic response to in-vitro stimulation by group A streptococcal M proteins of apparent virulence factor between IgAN, non-proliferative glomerulonephritis (NPGN), and normal subjects. M proteins were extracted from group A streptococcal strain type 5 and type 12 determined serologically.

Results. M protein-induced proliferation of lymphocytes from IgAN was higher than in NPGN but not in healthy control subjects. Flow cytometric analysis indicated that stimulation by M protein extracts derived from type 5 streptococci (M5) increased surface IgA-positive B cells in IgAN, but did not activate the production of soluble IgA. We also showed that M5 induced significant increases in TGF-ß, in culture supernatants of lymphocytes from patients with IgAN.

Conclusion. Our results suggest that Streptococcal infection may play an important role in the pathogenesis of IgAN by stimulating IgA production through TGF-ß synthesis.

Keywords: glomerulonephritis; IgA nephropathy; lymphocyte proliferation; streptococcal M protein; TGF-ß

Correspondence and offprint requests to: Yoshiyuki Ozono MD PhD, The Second Department of Internal Medicine, Nagasaki University School of Medicine, 7–1 Sakamoto 1 cho-me, Nagasaki-shi, Japan, 852–8501.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Wang, S. Li, P. J. Southern, and P. P. Cleary
Streptococcal modulation of cellular invasion via TGF-beta1 signaling
PNAS, February 14, 2006; 103(7): 2380 - 2385.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.