Nephrol Dial Transplant (2000) 15: 1638-1646
© 2000 European Renal Association-European Dialysis and Transplant Association
Role of complement and platelet-activating factor in the stimulation of phagocytosis and reactive oxygen species production during haemodialysis
1 Department of Nephrology, Dialysis and Transplantation, Hôpital Erasme, Université Libre de Bruxelles and 2 Department of Nephrology, Hôpital Universitaire Brugmann, Université Libre de Bruxelles, Belgium
Background. Neutrophil phagocytic functions have been studied extensively in haemodialysis (HD) patients; however, results are contradictory and the mechanisms that modulate phagocytosis and oxidative burst during dialysis are not completely understood.
Methods. The present study investigated neutrophil functions in a selected population of patients before and during clinical dialysis with cuprophane, and polyacrylonitrile (AN69) membranes. We measured phagocytosis of Escherichia coli and intracellular hydrogen peroxide (H2O2) production by flow cytometry in whole blood.
Results. Before dialysis, neutrophils from HD patients showed normal phagocytic capability and H2O2 formation. Phagocytosis of FITC-E. coli was significantly stimulated in cuprophane but not AN69-treated patients. Spontaneous and stimulated H2O2 production was enhanced with both cuprophane and AN69 membranes. We then investigated in vitro the role of complement and platelet-activating factor (PAF) in the activation of neutrophils. Incubation of whole blood with C5a increased phagocytosis but not H2O2 production. On the contrary, the addition of synthetic PAF showed a markedly stimulated H2O2 production without increase in phagocytosis. Moreover, during dialysis with formaldehyde-reused cuprophane, complement activation was abolished and phagocytosis was no longer enhanced, while the stimulation of H2O2 production persisted. In addition, we also excluded a particular role of the membrane itself in the activation of neutrophils.
Conclusion. We demonstrated that in a selected population of HD patients, neutrophils exhibit normal phagocytic capability and normal intracellular H2O2 production. During dialysis, the stimulation of phagocytosis observed with cuprophane is complement dependent, whereas the enhanced H2O2 production observed with both cuprophane and AN69 membranes might be related to PAF production.
Keywords: complement; dialysis membrane; HD; oxidative burst; PAF; phagocytosis
Correspondence and offprint requests to: Karine Gastaldello, Department of Nephrology, 808 Route de Lennik, B-1070 Brussels, Belgium.kgastald{at}ulb.ac.be
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Si, J. Behar, J. Wands, D. G. Beer, D. Lambeth, Y. Eugene Chin, and W. Cao STAT5 mediates PAF-induced NADPH oxidase NOX5-S expression in Barrett's esophageal adenocarcinoma cells Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G174 - G183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Banche, V. Allizond, F. Giacchino, N. Mandras, J. Roana, F. Bonello, P. Belardi, V. Tullio, C. Merlino, N. Carlone, et al. Effect of dialysis membrane biocompatibility on polymorphonuclear granulocyte activity in dialysis patients Nephrol. Dial. Transplant., December 1, 2006; 21(12): 3532 - 3538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. C. Grooteman, M. Gritters, I. M. P. M. J. Wauters, C. G. Schalkwijk, F. Stam, J. Twisk, P. M. ter Wee, and M. J. Nube Patient characteristics rather than the type of dialyser predict the variability of endothelial derived surface molecules in chronic haemodialysis patients Nephrol. Dial. Transplant., December 1, 2005; 20(12): 2751 - 2758. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Wu, J.-S. Chen, W.-M. Wu, T.-N. Liao, P. Chu, S.-H. Lin, C.-H. Chuang, and Y.-F. Lin Myeloperoxidase serves as a marker of oxidative stress during single haemodialysis session using two different biocompatible dialysis membranes Nephrol. Dial. Transplant., June 1, 2005; 20(6): 1134 - 1139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rysz, M. Kasielski, J. Apanasiewicz, M. Krol, A. Woznicki, M. Luciak, and D. Nowak Increased hydrogen peroxide in the exhaled breath of uraemic patients unaffected by haemodialysis Nephrol. Dial. Transplant., January 1, 2004; 19(1): 158 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Anding, P. Gross, J. M. Rost, D. Allgaier, and E. Jacobs The influence of uraemia and haemodialysis on neutrophil phagocytosis and antimicrobial killing Nephrol. Dial. Transplant., October 1, 2003; 18(10): 2067 - 2073. [Abstract] [Full Text] [PDF]
|
|