Nephrology Dialysis Transplantation, Vol 14, Issue 5 1176-1181, Copyright © 1999 by Oxford University Press
T Masaki, J Gilson, J Leypoldt and A Cheung
Background. Increases in plasma anaphylatoxins
frequently are used as an index of haemodialysis membrane biocompatibility;
however, their plasma levels may be influenced by the loss of
anaphylatoxins into the dialysate compartment. Methods.
We compared the generation and compartmental distribution of
anaphylatoxins, C3a and C5a, in a high flux and a low flux polysulfone
membrane dialyser when whole human blood was recirculated through an
in vitro haemodialysis circuit. Results.
Plasma C3a levels in high flux polysulfone (2.31±0.81
&mgr;g/ml) and low flux polysulfone (3.02±0.98
&mgr;g/ml) dialysers were comparable after 120 min
(P=NS). In contrast, dialysate C3a in high flux
polysulfone (0.65±0.31 &mgr;g/ml) accounted for
37.5±7.0% of the total detected (plasma+dialysate) C3a mass in
the dialysers, while dialysate C3a in low flux polysulfone dialysers
(0.01±0.01 &mgr;g/ml) accounted for only 0.3±0.3%
of the total mass (P<0.05; high flux
vs low flux). Anaphylatoxin C5a was undetectable in
the dialysate compartment of either dialyser examined.
Conclusions. Our results indicate that anaphylatoxins
readily traverse certain high flux dialysis membranes; consequently, plasma
C3a levels may not accurately reflect the C3-activating potential of these
membranes. Keywords: anaphylatoxins; biocompatibility;
complement; haemodialysis; membranes; permeability
ORIGINAL ARTICLES
Effect of permeability on indices of haemodialysis membrane biocompatibility
Medical and Research Services, Veterans Affairs Medical Center and Division of Nephrology and Hypertension, Department of Medicine, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; Corresponding author
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