Nephrology Dialysis Transplantation, Vol 14, Issue 1 70-75, Copyright © 1999 by Oxford University Press
R Thuraisingham and A Raine
Background: The nitric oxide system has been
implicated in several diseases with vascular complications including
diabetes mellitus and hypertension. Despite the high prevalence of
hypertension and cardiovascular complications in renal failure few studies
have examined vascular and endothelial function in uraemia. We therefore
chose to study possible abnormalities of the nitric oxide vasodilator
system in an animal model of chronic renal failure.
Methods: Adult spontaneous hypertensive rats and
Wistar Kyoto rats were subjected to a 5/6 nephrectomy with control animals
having sham operations. After 4 weeks blood pressure was recorded and the
animals were sacrificed. Branches of the mesenteric arteries were isolated
and mounted on a Mulvany myograph. All experiments were performed in the
presence of indomethacin (10-5 M). The vessels were
first preconstricted with noradrenaline exposed to increasing
concentrations of acetylcholine (10-8 to
10-4 M) and subsequently to sodium nitroprusside
(10-5). Results: There was no
difference in the relaxation of the four groups of vessels to any of the
concentrations of acetylcholine used nor was there any significant
difference in the EC50s (control Wistar Kyoto 6.1±1.4 x
10-8M; uraemic Wistar Kyoto 5.4±0.8 x
10-8M; control spontaneous hypertensive rats
4.5±0.6 x10-8M; uraemic spontaneous
hypertensive rats 6±0.7 x 10-8M).
Vasodilation in response to sodium nitroprusside was unchanged in uraemic
vessels. In addition the vascular responses to both acetylcholine and
sodium nitroprusside were unaltered in spontaneous hypertensive rats.
Conclusions: We conclude that normal agonist-induced
endothelium-dependent relaxation is maintained in experimental uraemia and
hypertension. Key words: chronic renal failure;
hypertension; myograph; nitric oxide
ORIGINAL ARTICLES
Maintenance of normal agonist-induced endothelium-dependent relaxation in uraemic and hypertensive resistance vessels
Anthony Raine Research Laboratory, St Bartholomew's Hospital, London, UK; Corresponding author at: Royal London Hospital, Whitechapel, London E1 1BB, UK
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