Nephrology Dialysis Transplantation, Vol 13, Issue 7 1745-1751, Copyright © 1998 by Oxford University Press
P Brunet, C Capo, J Dellacasagrande, X Thirion, J Mege and Y Berland
Purpose of study: IL-10 may explain the paradox
between immunodeficiency and oversecretion of cytokines in chronic
haemodialysis (HD) patients. We analysed the secretion of IL-10 by PBMC and
the expression of IL-10 mRNA in 10 long-term HD patients (108-276 months),
10 short-term HD patients (3-18 months), and 10 healthy controls.
Results: Spontaneous IL-10 secretion was higher in HD
patients than in controls (15 pg/mol vs 2 pg/ml),
P=0.001). It was detected in 13 of 20 patients and in 1 of 10 controls
(P=0.01). IL-10 mRNA expression was also higher in HD patients than in
controls. Spontaneous secretions of IL-10 and IL-6 were positively
correlated in patients. Il-10 secretion in response to LPS was higher than
the upper limit of control range in 4 of 10 long-term HD patients and in no
short-term HD patients (P=0.04). IL-10 mRNA expression was also higher in
long-term than in short-term HD patients. Conclusions:
This study demonstrates that IL-10 is spontaneously synthesized and
secreted in HD patients, supporting an immunomodulating role in this
setting. The greater IL-10-producing capacity in long-term HD patients
indicates a chronic effect of haemodialysis on PBMC responsiveness.
Key words: haemodialysis; immunodeficiency;
immunomodulation; interleukin-10; interleukin-6; peripheral blood
mononuclear cells
ORIGINAL ARTICLES
IL-10 synthesis and secretion by peripheral blood mononuclear cells in haemodialysis patients
Service de Néphrologie, Hôpital Sainte Marguerite, 270 Bd de Sainte-Marguerite, F-13009 Marseille, France; Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, Marseille, France; Service d'Information Médicale, Hôpital Sainte Marguerite, Marseille, France; Corresponding author
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