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Nephrology Dialysis Transplantation, Vol 13, Issue 2 277-282, Copyright © 1998 by Oxford University Press

ORIGINAL ARTICLES

Expression of TGF-{beta} receptors type I and II in human glomerulonephritis

A Muda, S Feriozzi, S Rahimi and T Faraggiana
Dipartimento di Medicina Sperimentale e Patologia, Universita 'La Sapienza', Sezione di Patologia Ultrastrutturale Viale Regina Elena, 324 (Policlinico), I-00161 Roma, Italy; Centro di Riferimento di Nefrologia e Dialisi, Azienda USL, Viterbo, Italy; Corresponding author

Background. Transforming growth factor-{beta} (TGF-{beta}) is a multipotent cytokine involved in the turnover of the extracellular matrix. Signal transduction of TGF-{beta} is regulated via at least five different surface receptors; most of the effects, however, are mediated through the interaction of receptors type I and II (RI and RII). We investigated the glomerular expression of TGF-{beta} and its receptors RI and RII in human glomerulonephritis. Methods. Indirect immunofluorescence using monoclonal and polyclonal antibodies against TGF-{beta} isoforms (1,2,3 and 2,3), TGF{beta}-RI and TGF{beta}-RII has been carried out on 30 consecutive renal biopsies (5 FSGS, 11 IgAN, 4 MCGN, 6 SLE, 2 RPGN) and on normal kidney tissue. Results. Glomerular immunoreactivity for TGF-{beta} isoforms correlated with the severity of proliferative lesions: immunofluorescent signal was absent or tracelike in normal kidneys, FSGS, and IgAN with mild mesangial cellularity, and was highest in IgAN with severe mesangial proliferation., MPGN, RPGN, and SLE. Glomerular positivity for TGF-{beta}-RI and -RII was detectable in SLE and RPGN; a low signal was present also in MPGN. Other types of glomerulonephritis, including focal extracapillary proliferations, and glomeruli of normal kidneys were always negative. Conclusions. Our data show that TGF-{beta} expression is a good indicator of the severity of proliferative glomerular lesions in most, if not all cases and that RI-RII expression occurs at levels detectable with indirect immunofluorescence in limited number of glomerulonephritides, mostly associated with systemic diseases. A complex interaction between TGF-{beta} and its ligand may represent a critical factor conditioning the tissue response to immunological injury. Keywords: human glomerulonephritis; immunohistochemistry; TGF-{beta}; TGF-{beta} receptors
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