Nephrology Dialysis Transplantation, Vol 12, Issue 6 1137-1142, Copyright © 1997 by Oxford University Press
G Hillis, P Roy-Chaudhury, L Duthie, K Stewart, P Brown, J Simpson and A MacLeod
Aim. To compare the expression of {beta}1 integrins
in renal biopsies from patients with IgA nephropathy with that found in
normal human kidney. Methods. Thirty renal biopsies
from patients with IgA disease plus six control specimens were stained with
monoclonal antibodies directed against the &agr;1, &agr;2,
&agr;3, &agr;4, &agr;6, &agr;v, and {beta}1 integrin
chains using the alkaline phosphatase anti-alkaline phosphatase (APAAP)
technique. The intensity of integrin expression was graded
semiquantitatively by a pathologist unaware of the antibody used.
Results. Glomerular crescents stained strongly for
&agr;3, &agr;v, and {beta}1, but integrin expression was greatly
reduced or absent in fibrotic glomeruli. There were no alterations in the
intensity of mesangial cell staining for any of the integrins tested. There
was accentuated staining for the &agr;2, &agr;5, &agr;v, and
{beta}1 chains in areas of interstitial scarring plus &agr;2,
&agr;3, &agr;v, and {beta}1 on damaged tubules. Inflammatory
cells expressed &agr;4, &agr;5, and {beta}1.
Conclusions. In IgA nephropathy the interstitium is
the main site of altered {beta}1 integrin expression. Glomerular
crescents also express several {beta}1 integrins, but we found no
differences in the intensity of integrin expression on mesangial cells.
Altered {beta}1 integrin expression may play a role in
tubulointerstitial scarring in IgA disease. Thus modulation of integrin
expression might attenuate this process. Keywords:
{beta}1 integrins; glomerulonephritis; IgA nephropathy;
fibrosis
ORIGINAL ARTICLES
Expression of {beta}1 integrins in IgA nephropathy
Departments of Medicine & Therapeutics and Pathology, University of Aberdeen, Aberdeen, UK; Division of Clinical Immunology Beth Israel Hospital, Boston, USA; Corresponding author address: Department of Cardiology, The Royal Infirmary of Edinburgh, Lauriston Place, Edinburgh, EH3 UK, UK
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