Nephrology Dialysis Transplantation, Vol 12, Issue 11 2339-2348, Copyright © 1997 by Oxford University Press
J Filho, P Barany, P Stehle, P Furst and J Bergstrom
Background: Disturbances in amino acid (AA) metabolism
in uraemia have mainly been reported to occur in plasma and muscle. The
erythrocytes (RBC) constitute a large proportion of the free AA in blood
and may play an important role in the interorgan transport of AA. This
report presents the first data on AA levels obtained simultaneously from
three different compartments in uraemic patients.
Method: Muscle biopsy and blood samples were obtained
from 38 haemodialysis (HD), 22 continuous peritoneal dialysis (CPD) and 10
end-stage renal failure patients for determination of free amino acids by
reversed-phase HPLC. The results are compared to data obtained from 27
health subjects under the same conditions. Results:
For a number of non-essential AA (alanine, glycine, asparagine, arginine)
and for lysine, elevated concentrations were present simultaneously in RBC
and in muscle but not in plasma. On the other hand, low concentrations of
some essential AA (leucine, valine, phenylalanine, tyrosine) were observed
in RBC and in plasma, while the concentrations in muscle were normal. Most
of the non-essential AA (NEAA), especially taurine and glutamine, had much
higher muscle/plasma gradients than RBC/plasma gradients, although an
accumulation in RBC of glycine, serine, arginine, asparagine, ornithine,
glutamate and taurine was observed. Most of the essential AA (EAA) showed
higher muscle/plasma gradients, whereas the RBC/plasma gradients were
approximately 1.0. Conclusion: Our findings are in
agreement with studies that have shown that RBC and plasma play independent
and opposing roles in AA interorgan transport. The results indicate that
there are several AA abnormalities in all three compartments in uraemic
patients. They also suggest that there may be some specific common changes
of selected transport systems for both RBC and muscle in uraemia.
Determination of AA in RBC should be considered when undertaking metabolic
and clinical studies of AA disturbances. Key words:
amino acid; dialysis; erythrocytes; muscle; plasma; uraemia
ORIGINAL ARTICLES
Free amino-acid levels simultaneously collected in plasma, muscle, and erythrocytes of uraemic patients
Division of Renal Medicine and Baxter Novum, Department of Clinical Science, Karolinska Institutet, Huddinge University Hospital, Stockholm, Sweden; Department of Nutritional Biochemistry, Hohenheim University, Stuttgart, Germany; Corresponding author at: Department of Renal Medicine, Huddinge University Hospital K56, S-141 86 Huddinge, Sweden
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