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NDT Advance Access originally published online on October 4, 2005
Nephrology Dialysis Transplantation 2005 20(12):2598-2601; doi:10.1093/ndt/gfi176
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© The Author [2005]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

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Messenger RNA assessment in clinical nephrology: perspectives and progress of methodology

Michael Eikmans, Hans J. Baelde, Emile de Heer and Jan A. Bruijn

Department of Pathology, Leiden University Medical Center, The Netherlands

Correspondence and offprint requests to: Michael Eikmans, Department of Pathology, Leiden University Medical Center, The Netherlands. Email: m.eikmans@lumc.nl

Keywords: kidney disease; laser-capture microdissection; messenger RNA; prognosis; real-time PCR; RNA extraction

The first 10% of the full text of this article appears below.



   Introduction
 
Molecular biology offers new opportunities for clinical medicine. A promising clinical application of molecular biology is the identification of mRNA expression patterns in diseased organs. These mRNA expression patterns may provide information regarding diagnosis, prognosis and responsiveness to treatment. The development of technologies such as microarray analysis and real-time PCR enables the study of gene expression networks in renal biopsies. This fact, in combination with the usage of laser-capture microdissection, enables gene expression analysis in a nephron segment-specific way. We will elaborate on the potential applications of mRNA assessment in clinical nephrology. Progress in the optimization of protocols for acquiring adequate renal tissue and intact RNA will be discussed.



   Perspectives of mRNA assessment in clinical practice
 
The question of why it is useful to analyse levels of mRNA in renal biopsies must be answered. Firstly, mRNA quantitation could be used as a diagnostic tool. In renal pathology, it is sometimes difficult to formulate a diagnosis . . . [Full Text of this Article]



   Toward an optimal protocol for the acquisition of intact RNA from renal tissue
 


   Conclusion
 

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