NDT Advance Access originally published online on March 19, 2004
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Nephrol Dial Transplant (2004) 19: 1347-1351
Nephrol Dial Transplant Vol. 19 No. 6 © ERA-EDTA 2004; all rights reserved
Editorial Comment
Gene expression analysis in renal biopsies
Department of Nephrology, Medizinische Poliklinik, University of Munich, Germany
Correspondence and offprint requests to: Matthias Kretzler MD, Med. Poliklinik, Innenstadt, Ludwig Maximilians University of Munich, Pettenkoferstrasse 8a, D-80336 München, Germany. Email: kretzler@med-poli.med.uni-muenchen.de
Keywords: cDNA array; gene expression; kidney; microdissection; renal biopsy; RT–PCR
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Introduction |
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Fine needle kidney biopsy with subsequent analysis of the tissue core by light microscopy and immunhistology is currently the diagnostic gold standard in nephrology [1]. The addition of immunofluorescence and electron microscopic techniques into routine biopsy processing enabled a further refinement of the diagnostic categories used [2]. Despite recent developments in molecular biology most intrinsic renal diseases are still of unknown aetiology and are classified according to descriptive diagnostic criteria resulting in treatment with non-specific therapies.
The human genomic information now available opens a wide range of new opportunities in biomedicine [3,4]. A comprehensive description of mRNA levels encoding functionally relevant molecules could play an important role in molecular analysis and understanding of human disease [5]. Identification of specific mRNA expression pattern in diseased tissues and their correlation with diagnosis, prognosis and response to the different treatments available could be
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The ERCB project: goals and principles |
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Sample collection, preparation and expression profiling
Real-time RT–PCR
Oligonucleotide or cDNA arrays
Laser-microdissection and mRNA quantification of routine processed renal biopsies
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Application of molecular diagnostics and gene expression analysis |
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Outlook and conclusion |
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