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NDT Advance Access published online on October 10, 2008
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfn559
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© The Author [2008]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org


Improved preservation and microcirculation with POLYSOL after transplantation in a porcine kidney autotransplantation model

Marie-Claire J. M. Schreinemachers1, Benedict M. Doorschodt1, Sandrine Florquin2, Marius A. van den Bergh Weerman2, Johannes B. Reitsma3, Wei Lai4, Mario Sitzia5, Thomas M. Minor4, Rene H. Tolba5,6* and Thomas M. van Gulik1,*

1 Surgical Laboratory 2 Department of Pathology 3 Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands 4 Surgical Research Division, Department of Surgery 5 House of Experimental Therapy, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn 6 Institute for Laboratory Animal Science and Experimental Surgery, RTWH-Aachen University, Pauwelsstr. 30, D-52074 Aachen, Germany

Correspondence and offprint requests to: Rene H. Tolba, Institute for Laboratory Animal Science and Experimental Surgery, RTWH-Aachen University, Pauwelsstr. 30, D-52074 Aachen, Germany. Tel: +49-241-80-80472; Fax: +49-241-80-82462; E-mail: rtolba{at}ukaachen.de



  Abstract

Background. The most widely used preservation method for kidney grafts is cold static storage (CS) using the University of Wisconsin (UW) solution. To date, new preservation solutions have not been able to significantly improve preservation quality of grafts. The aim of this study was to compare POLYSOL, a recently developed low viscosity preservation solution, and the UW solution for CS of porcine kidney grafts.

Methods. In a porcine autotransplantation model, real-time parameters of the renal microcirculation were evaluated using the novel oxygen-to-see (O2C) combined laser Doppler and flowmetry system. Thereafter, kidneys were retrieved and washed out with POLYSOL or UW followed by 20-h CS. After the preservation period, the contralateral kidneys were removed and the preserved kidneys autotransplanted. The microcirculation was re-assessed at 10 min after reperfusion and at 7 days posttransplant, prior to removal of the grafts for histological evaluation.

Results. POLYSOL was able to better preserve the microcirculation compared to UW as expressed by higher values of capillary blood flow, blood flow velocity and tissue oxygen saturation values. In addition, CS using POLYSOL resulted in improved functional recovery demonstrated by lower posttransplant serum creatinine and blood urea values in comparison to the UW group. Also, structural integrity was better preserved in the POLYSOL group, compared to UW.

Conclusions. This study in a clinically relevant large animal model showed that a new preservation solution, POLYSOL, resulted in improved preservation quality of kidney grafts compared to the UW solution.

Keywords: kidney; microcirculation; pig; preservation solution; transplantation

* These authors contributed equally to this work.

Received for publication: 10. 6.08
Accepted in revised form: 12. 9.08


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