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NDT Advance Access published online on April 19, 2008
Nephrology Dialysis Transplantation, doi:10.1093/ndt/gfn212
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© The Author [2008]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org


Proteomic profiling and identification in peritoneal fluid of children treated by peritoneal dialysis

Renske Raaijmakers1, Wendy Pluk2, Cornelis H. Schröder3, Jolein Gloerich2, Elisabeth A.M. Cornelissen1, Hans J.C.T. Wessels4, Johannes L. Willems5, Leo A.H. Monnens1 and Lambert P.W.J. van den Heuvel1,2,4

1 Department of Paediatric Nephrology 2 Laboratory of Paediatrics and Neurology, Nijmegen Proteomics Facility, Radboud University Nijmegen Medical Centre, Nijmegen 3 Department of Paediatric Nephrology, University Medical Centre Utrecht, Utrecht 4 Nijmegen Centre for Mitochondrial Disorders 5 Department of Clinical Chemistry, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

Correspondence and offprint requests to: Renske Raaijmakers, Paediatric Nephrology 833, UMC St Radboud, PO Box 9101, 6500 HB Nijmegen, The Netherlands. Tel: +31-24-3666292; Fax: +31-24-3619348; E-mail: r.raaijmakers{at}cukz.umcn.nl



  Abstract

Background. Proteomic technologies offer a high-throughput analysis of the expression of proteins in biological samples. The global analysis of the proteins in peritoneal dialysis (PD) fluid will provide a better understanding of the biological processes of the peritoneal membrane.

Methods. The dialysate of nine paediatric PD patients was collected from peritoneal equilibrium tests with 3.86% glucose. Proteins were separated on a 10% SDS–PAGE gel and in-gel digested with trypsin. Peptide mixtures were analysed using nanoLC-MS/MS and results were searched against the NCBI database.

Results. A total number of 189 proteins were identified in the PD fluid of nine patients, with 88 proteins shared by all patients. These 88 proteins accounted for 47% of the identified proteins and >90% of the total protein content in the analysed samples. Proteins were subdivided into eight different classes according to function.

Conclusions. This study gives a representative overview of the proteins present in PD fluid. The proteins in PD fluid reflect plasma proteins as well as local peritoneal processes. Potentially interesting proteins are revealed.

Keywords: mass spectrometry; peritoneal dialysis; proteomics; paediatric

Received for publication: 5.12.07
Accepted in revised form: 21. 3.08


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