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Nephrol Dial Transplant (1993) 8: 1223-1227
© 1993 European Renal Association-European Dialysis and Transplant Association

research-article

Adherence of human monocytes to haemodialysis membranes

S. David1, C. Tettaz2, G. Camussi3, F. Canino1, C. Almici4, V. Rizzoli4 and V. Cambi1,

1Cattedra di Nefrologia, Università di Parma Parma, Italy 2Bellw Research Laboratories Bellco S.p.A., Mirandola (Modena), Italy 3Cattedra di Nefrologia, Università di Napoli Napoli, Italy 4Cattedra di Ematologia, Università di Parma Parma, Italy

Correspondence and offprint requests to: Correspondence and offprint requests to: V. Cambi MD, Cattedra di Nefrologia, University of Parma, Via Gramsci 14, 43100 Parma, Italy

In the present study we evaluated spontaneous and stimulated adherence of human monwytes to regenerated cellulose and polyacrylonitrile (AN69) membranes. Spontaneous adherence at 60 min was significantly higher for regenerated cellulose (28±2%, P<0.001) than for AN69 (11±2) membranes. Stimuli such as bacterial lipopolysaccharide, TNF{alpha}, interleukin-1 and -6 as well as platelet-activating factor, but not IL4, significantly enhanced adherence at 60 min to AN69 (28 to 30%). In contrast, adherence was not further inducible in the presence of regenerated cellulose. Both spontaneous and cytokine/bacterial lipopolysaccharide-stimulated adherence were significantly reduced by SDZ-63072, a specific plateletactivating factor receptor antagonist. This difference in sensitivity of monocyte adherence reflects probably the intrinsic ability of regenerated cellulose to provide maximal spontaneous monocyte adhesion. These data suggest that PAF may act as an adherence mediator. This is in line with the ability of regenerated cellulose to directly stimulate monocytes to synthesize plateletactivating factor and with the ability of cytokines and bacterial lipopolysaccharide to stimulate its synthesis. Although AN69 has a low adherence potential, bacterial lipopolysaccharide or cytokines may blunt the biocompatibility of this membrane.

Keywords: adherence; biocompatibility; cytokines; monwytes; PAF


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