NDT Advance Access originally published online on April 15, 2009
Nephrology Dialysis Transplantation 2009 24(9):2708-2713; doi:10.1093/ndt/gfp169
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Cervical heterotopic kidney transplantation in rats using non-suturing and preserving-bag techniques
1 Department of Physiology, Dalian Medical University, Dalian, Liaoning Province 116044 2 Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu Province 210002 3 College of Health Administration 4 Division of Organ Transplantation, Dalian Medical University, Dalian, Liaoning Province 116027, China
Correspondence and offprint requests to: Yuan Zou; E-mail: zhuliang0210{at}sina.com
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Abstract |
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Background. This study describes a simple and stable cervical heterotopic kidney transplantation method in rats that uses artery sleeve anastomosis, vein cuff anastomosis and preserving-bag techniques.
Methods. The donor graft, consisting of kidney, renal vein (RV), renal artery (RA) and a ureterocystic flap, was removed en bloc and perfused in situ. The donor RA was end-to-end anastomosed to the recipient left common carotid artery (CCA) using a sleeve anastomosis, and the donor RV was connected to the recipient right external jugular vein (EJV) using a cuff technique. During the vascular anastomosis, the kidney graft was placed in a lactated Ringer's solution ice-water preserving bag. The donor bladder patch was exteriorized to form cervical cutaneous stoma.
Results. A total of 104 heterotopic renal transplantations were performed, which included pre-experimental (62 operations) and experimental stages (42 operations). The success rates of the two stages were 80.6% and 95.2%, respectively. The time for surgery was 40 ± 6 min, the average time for donor surgery was 20 ± 5 min, the preparation time for the graft was 8 ± 2 min, the operative time for the recipient was 18 ± 3 min that included the time for the arterial anastomosis (5 ± 2 min) and venous anastomosis (2 ± 1 min), the cold ischaemic time of the graft was 15 ± 3 min and the warm ischaemic time of the graft was 2 ± 1 min.
Conclusion. We developed an easy and reliable model of cervical heterotopic kidney transplantation that may provide a useful tool for investigating kidney transplantation rejection and retransplantation pathology.
Keywords: animal models; cuff technique; heterotopic; kidney transplantation; sleeve anastomosis
Received for publication: 17. 7.08
Accepted in revised form: 23. 3.09