NDT Advance Access originally published online on May 31, 2005
Nephrology Dialysis Transplantation 2005 20(9):1922-1931; doi:10.1093/ndt/gfh913
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Albumin and hydroxyethyl starch 130 kDa/0.4 improve filter clearance and haemocompatibility in haemo- and plasmafiltration—an in vitro study
1 Department of Anaesthesiology and 2 Department of Clinical Chemistry, University Hospital Aachen, Rheinisch-Westfälische Technische Hochschule Aachen, Aachen, Germany, 3 Department of Comparative Medicine and Experimental Animal Sciences and 4 Department of Paediatric Pneumology and Immunology, Charité, Campus Virchow, Humboldt-University, Berlin, Germany
Correspondence and offprint requests to: Juliane K. Unger, Department of Comparative Medicine and Experimental Animal Sciences, Charité Campus Virchow Hospital, Augustenburger Platz 1, Forum 4, D-13353 Berlin, Germany. Email: juliane.unger{at}charite.de
Background. Apart from their standard applications, haemofiltration (HF) and plasmafiltration (PF) may provide helpful therapy for sepsis, multiple organ- and acute liver-failure. Some colloids cause either decreases or increases in blood cell agglomeration. We hypothesized that solutions which reduce cell aggregability may lead to both improved filter clearance and better haemocompatibility due to decreasing rates of clogged hollow fibres.
Methods. Heparinized porcine blood (5 IU/ml) was used in an in vitro circuit. The filter types tested were from GAMBRO: HF66D (effective membrane surface: 0.6 m2) and PF1000N (effective membrane surface: 0.15 m2). Albumin (ALB), hydroxyethyl starch (HES) 200/0.5, HES 130/0.4, gelatin (GEL) or normal saline (0.9%) were added to the blood (n = 6/group). Recirculation systems were run for 2 h. Spontaneous haemolysis and filter resistance >420 mmHg were selected as indications of maximal flow rates. Sieving coefficients were determined for 17 parameters at the lowest and highest blood flows and filtration rate.
Results. Based on the filter types used, supplementation of ALB and HES130/0.4 led to an improved filter clearance without increasing the number of clogged capillary membranes or causing impaired haemocompatibility. Sieving coefficients for most solutes were independent of volume substitute and flow rate. Haemocompatibility and filter clearance deteriorated after addition of HES200 or GEL to the blood.
Conclusions. Under standardized in vitro conditions, we found that colloids which reduce cell aggregability cause improved HF- and PF-performance. This phenomenon may provide new options for higher clearances and may lead to new concepts in low dose anticoagulation.
Keywords: clearance; colloids; crystalloids; haemocompatibility; haemofiltration; plasmapheresis
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