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Nephrol Dial Transplant (2001) 16: 3-13
© 2001 European Renal Association-European Dialysis and Transplant Association


Development and characterization of novel erythropoiesis stimulating protein (NESP)

Reprinted with permission from the British Journal of Cancer (2001), 84(8) supplement 1. © 2001 Cancer Research Campaign.

Joan C. Egrie and Jeffrey K. Browne

Amgen Inc., 1 Amgen Center Drive, MS 27-4-A, Thousand Oaks, CA 91320-1799, USA

Abstract

Studies on human erythropoietin (EPO) demonstrated that there is a direct relationship between the sialic acid-containing carbohydrate content of the molecule and its serum half-life and in vivo biological activity, but an inverse relationship with its receptor binding affinity. These observations led to the hypothesis that increasing the carbohydrate content, beyond that found naturally, would lead to a molecule with enhanced biological activity. Hyperglycosylated recombinant human EPO (rHuEPO) analogues were developed to test this hypothesis. Darbepoetin alfa (novel erythropoiesis stimulating protein, NESP), which was engineered to contain five N-linked carbohydrate chains (two more than rHuEPO), has been evaluated in preclinical animal studies. Due to its increased sialic acid-containing carbohydrate content, NESP is biochemically distinct from rHuEPO, having an increased molecular weight and greater negative charge. Compared with rHuEPO, it has an approximately 3-fold longer serum half-life, greater in vivo potency, and can be administered less frequently to obtain the same biological response. NESP is currently being evaluated in human clinical trials for treatment of anaemia and reduction in its incidence.

Keywords: biological activity; carbohydrate; darbepoetin alfa; erythropoietin; pharmacokinetics; review

Notes

Correspondence and offprint requests to: Dr Joan C. Egrie, Amgen Inc., 1 Amgen Center Drive, MS 27-4-A, Thousand Oaks, CA 91320-1799, USA.


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