Nephrol Dial Transplant (2001) 16: 27-33
© 2001 European Renal Association-European Dialysis and Transplant Association
Angiotensin II activates nuclear transcription factor-
B in aorta of normal rats and in vascular smooth muscle cells of AT1 knockout mice
Laboratory of Vascular and Renal Pathology, Fundación Jiménez Díaz, Universidad Autónoma, Madrid, Spain
Background. Nuclear factor-
B (NF-B) regulates many genes involved in vascular physiopathology. Angiotensin II (Ang II) participates in the pathogenesis of several cardiovascular diseases. In a model of atherosclerosis, we have noted NF-B activation in the neointimia lesion that was decreased by angiotensin-converting enzyme (ACE) inhibitors. However, the potential direct effect of Ang II in the vascular activation of NF-B has not been completely elucidated.
Methods. We first investigated whether Ang II elicited in vivo activation of NF-B in large vessels of normal rats by systemic infusion of Ang II (50 ng/kg/min, s.c.) into rats for 72 h. In order to investigate the receptor involved in this process, we also studied the direct effect of Ang II on cultured vascular smooth muscle cells (VSMCs) from wild-type and AT1 knockout mice.
Results. Ang II-infused rats showed activated NF-B in the endothelial and vascular smooth muscle cells of the aorta (southwestern histochemistry). In cultured VSMCs from wild-type mice, Ang II increased NF-B activity that was partially inhibited by AT1 (losartan) and AT2 (PD123319) antagonists. In VSMC from AT1 knockout mice, Ang II also activated NF-B.
Conclusions. These data show that Ang II via AT1 and AT2 activates NF-B in vascular cells both in vivo and in vitro, and suggest a potential involvement of the AT2 receptor in the pathogenesis of vascular diseases, including hypertension and atherosclerosis.
Keywords: angiotensin II; AT1 knockout mice; NF- B; vascular cells
Correspondence and offprint requests to: Marta Ruiz-Ortega, PhD, Laboratory of Renal and Vascular Pathology, Fundación Jiménez Díaz, Avda Reyes Católicos, 2, 28040 Madrid, Spain.
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