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Nephrol Dial Transplant (2001) 16: 1598-1606
© 2001 European Renal Association-European Dialysis and Transplant Association

Conjugated dienes: a critical trait of lipoprotein oxidizability in renal fibrosis

Bruno Poirier1, Odile Michel1, Raymond Bazin2, Jean Bariéty1, Jacques Chevalier1,, Isaac Myara1,3 and with the technical assistance of Anh-Thu Gaston,1

1 INSERM U 430, Broussais Hospital and Claude Bernard Association, 2 INSERM U 465, Institut des Cordeliers, Paris, and 3 Laboratory of Applied Biochemistry, Faculty of Pharmaceutical and Biological Sciences, Châtenay-Malabry, France

Background. We assessed whether a differential oxidizability of apolipoprotein B (apo B)-containing lipoproteins (LDL and VLDL) may explain the oxidative stress that we had observed at the onset of renal fibrosis in Zucker obese (ZO) rats (Nephrol Dial Transplant 2000, 15: 467–476).

Methods. Ex vivo copper-induced oxidation of lipoproteins was performed in 1-, 3-, and 9-month-old ZO and age-matched lean (ZL) rats. LDL/VLDL oxidizability was determined by spectrophotometry at 234 nm by monitoring the formation of conjugated diene hydroperoxides.

Results. A significant increase in lag time (reflecting the resistance to oxidation) was observed in ZO rats at 3 months while the maximal diene production (reflecting the amount of hydroperoxides formed during oxidation) was higher in ZO than in ZL rats as early as 1 month. Lipoproteins were larger in ZO than in ZL rats, as shown by their core to surface component ratio. Furthermore, ZO lipoproteins had increased vitamin E and polyunsaturated fatty acid (PUFA) content, with no change in vitamin E/PUFA ratio.

Conclusions. Rather than oxidizability of apo B-containing lipoproteins, the ability of these molecules to produce high levels of conjugated dienes, which can act as toxic tissue messengers, appears to be a critical trait in the development of renal fibrosis in this rat model of obesity and renal fibrosis.

Keywords: glomerulosclerosis; kidney lesion; LDL/ VLDL; obesity; oxidative stress; Zucker rat

Correspondence and offprint requests to: Dr Jacques Chevalier, Immunopathologie Rénale et Vasculaire, INSERM U 430, Hôpital Broussais, 96 Rue Didot, 75674 Paris Cedex 14, France.


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