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Nephrol Dial Transplant (2001) 16: 552-555
© 2001 European Renal Association-European Dialysis and Transplant Association

Intraplatelet calcium levels in patients with acute renal failure before and after the administration of loop diuretics

Ilona R. Shilliday1, and Marjorie E. M. Allison1,2

1 Renal Unit, Glasgow Royal Infirmary 2 Department of Medicine, University of Glasgow, Scotland, UK

Background. Intracellular calcium [Ca]i has been found to be elevated in hypoxic cells in vitro and in erythrocytes and lymphocytes from patients who are septic. Loop diuretics decrease [Ca]i in platelets from patients with hypertension and in red blood cells from normal volunteers. We report the results of a study designed to measure [Ca]i in platelets from patients with acute renal failure (ARF) before and after the administration of loop diuretics.

Methods. Sixteen healthy adults and seven patients with ARF were enrolled into the study. Intraplatelet calcium was measured using a fluorescent probe (quin2). Patients with ARF all received intravenous (i.v.) dopamine, 2 µg/kg body weight, and 20% mannitol, 100 ml every 6 h and, in double-blind manner, either torasemide, frusemide, or placebo, 3 mg/kg body weight i.v. every 6 h. Data from subjects given either frusemide or torasemide have been considered together and termed the diuretic group.

Results. Basal levels of [Ca]i in platelets from patients with ARF were significantly higher than in controls (126.9±35.7 nmol/l vs 85.7±22.2 nmol/l, P=0.02), but were not affected by the administration of loop diuretic (126.9±35.7 nmol/l vs 165.9±49.7 nmol/l, P=0.09, pre- vs post-diuretic).

Conclusions. Intraplatelet calcium is raised in patients with ARF. Loop diuretics have no significant effect on intraplatelet calcium in these patients.

Keywords: acute renal failure; arginine vasopressin; dopamine; intraplatelet calcium; loop diuretics; mannitol; quin2

Correspondence and offprint requests to: Dr I. R. Shilliday, Renal Unit, Western Infirmary, Glasgow G11 6NT, UK.


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Home page
 Nephrol Dial TransplantHome page
W. H. Horl and I. Shilliday
Intraplatelet calcium levels in patients with acute renal failure
Nephrol. Dial. Transplant., November 1, 2001; 16(11): 2275 - 2276.
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