Nephrology Dialysis Transplantation, Vol 14, Issue 90001 14-16, Copyright © 1999 by Oxford University Press
E De Heer, L Aaldering and S Florquin
Chronic graft-vs-host disease (GvH), induced by
injection of DBA/2 lymphocytes into (C57BL/6 x DBA/2)F1 hybrids, is a
murine model for lupus nephritis, associated with a Th2-dependent
polyclonal B cell activation. The development of glomerulosclerosis in this
model is preceded by a glomerular influx of LFA-1+ T
cells. We investigated whether exposure to bacterial superantigen would
modulate the course of this autoimmune syndrome. Injection of the bacterial
superantigen staphylococcal enterotoxin B (SEB) in mice has been shown to
induce the activation of TcRV{beta}8+ T cells.
Within 2 weeks after GvH induction, mice were injected twice with 20
&mgr;g of SEB and the following parameters were examined: cytokine and
Ig profile, proteinuria and renal pathology. The second SEB injection
induced in GvH mice an increased release of both interferon-&ggr;
(IFN-&ggr;) and interleukin-10 (IL-10) as compared with control F1
mice. No differences were observed in IL-2 production. SEB-treated GvH mice
demonstrated a delayed onset of proteinuria. Histological analysis of the
kidney showed that SEB-challenged GvH mice displayed significantly more
interstitial inflammation and mesangial proliferation together with more
IgG2a deposits in glomeruli than non-injected GvH mice. From these results,
we conclude that GvH mice are more responsive to SEB in terms of cytokine
production and that bacterial infection can modulate the course of this
renal disease from a membranous to a more proliferative type of
nephropathy.Keywords:
graft-vs-host disease; lupus nephritis;
staphylococcal enterotoxin G; superantigen; cytokines
ORIGINAL ARTICLES
T cell subsets in experimental lupus nephritis: modulation by bacterial superantigen
Leiden University Medical Center, Department of Pathology, LI-Q, PO Box 95600, 2300 RC, Leiden, The Netherlands; Corresponding author
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