Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (74)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Furness, P.
Right arrow Articles by Pringle, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furness, P.
Right arrow Articles by Pringle, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nephrology Dialysis Transplantation, Vol 14, Issue 5 1234-1237, Copyright © 1999 by Oxford University Press

PRELIMINARY REPORTS

Glomerular expression of nephrin is decreased in acquired human nephrotic syndrome

P Furness, L Hall, J Shaw and J Pringle
Department of Pathology, Leicester General Hospital, Gwendolen Road, Leicester LE5 4PW, UK; Corresponding author

Background. Nephril recently has been identified as a putative adhesion molecule, expressed in the glomerulus, in which mutations cause congenital nephrotic syndrome of Finnish type. We sought to determine whether expression of nephrin is altered in human glomeruli in patients with acquired nephrotic syndrome. Methods. We performed PCR amplification of nephrin cDNA, using cDNA previously prepared from single human glomeruli plucked fresh from the surface of human renal biopsies. We had available four cases of nephrotic syndrome (one membranous, three minimal change) and six normal controls. PCR product quantitation was by gel densitometry, confirmed by enzyme-linked immunosorbent assay using a specific oligonucleotide probe. Results were corrected for reaction efficiency and glomerular cellularity by expression as a ratio to levels of the 'housekeeping gene' glyceraldehyde phosphate dehydrogenase. Results. Glomerular levels of nephrin mRNA are significantly decreased in cases of minimal change nephrotic syndrome. An apparent reduction was also seen in the single case of membranous nephropathy which was available for study. Conclusions. Abnormalities of nephrin expression appear to be associated with acquired as well as congenital causes of human nephrotic syndrome. Keywords: adhesion; glomerulus; nephrin; nephrotic syndrome; slit diaphragm
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
M. Okamura, Y. Takano, Y. Saito, J. Yao, and M. Kitamura
Induction of nephrin gene expression by selective cooperation of the retinoic acid receptor and the vitamin D receptor
Nephrol. Dial. Transplant., October 1, 2009; 24(10): 3006 - 3012.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
F. Duner, J. Patrakka, Z. Xiao, J. Larsson, A. Vlamis-Gardikas, E. Pettersson, K. Tryggvason, K. Hultenby, and A. Wernerson
Dendrin expression in glomerulogenesis and in human minimal change nephrotic syndrome
Nephrol. Dial. Transplant., August 1, 2008; 23(8): 2504 - 2511.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
S. H. Han, S. Y. Park, J.-J. Li, S. J. Kwak, D. S. Jung, H. Y. Choi, J. E. Lee, S. J. Moon, D. K. Kim, D.-S. Han, et al.
Glomerular glucocorticoid receptor expression is reduced in late responders to steroids in adult-onset minimal change disease
Nephrol. Dial. Transplant., January 1, 2008; 23(1): 169 - 175.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
A. Nakajo, J. Khoshnoodi, H. Takenaka, E. Hagiwara, T. Watanabe, H. Kawakami, R. Kurayama, Y. Sekine, F. Bessho, S. Takahashi, et al.
Mizoribine Corrects Defective Nephrin Biogenesis by Restoring Intracellular Energy Balance
J. Am. Soc. Nephrol., September 1, 2007; 18(9): 2554 - 2564.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. Takano, K. Yamauchi, N. Hiramatsu, A. Kasai, K. Hayakawa, M. Yokouchi, J. Yao, and M. Kitamura
Recovery and maintenance of nephrin expression in cultured podocytes and identification of HGF as a repressor of nephrin
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1573 - F1582.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
N. Miyauchi, A. Saito, T. Karasawa, Y. Harita, K. Suzuki, H. Koike, G. D. Han, F. Shimizu, and H. Kawachi
Synaptic Vesicle Protein 2B Is Expressed in Podocyte, and Its Expression Is Altered in Proteinuric Glomeruli
J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2748 - 2759.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. V. Cybulsky, R. J. Quigg, and D. J. Salant
Experimental membranous nephropathy redux
Am J Physiol Renal Physiol, October 1, 2005; 289(4): F660 - F671.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. Koshikawa, M. Mukoyama, K. Mori, T. Suganami, K. Sawai, T. Yoshioka, T. Nagae, H. Yokoi, H. Kawachi, F. Shimizu, et al.
Role of p38 Mitogen-Activated Protein Kinase Activation in Podocyte Injury and Proteinuria in Experimental Nephrotic Syndrome
J. Am. Soc. Nephrol., September 1, 2005; 16(9): 2690 - 2701.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
B. Bussolati, M. C. Deregibus, V. Fonsato, S. Doublier, T. Spatola, S. Procida, F. Di Carlo, and G. Camussi
Statins Prevent Oxidized LDL-Induced Injury of Glomerular Podocytes by Activating the Phosphatidylinositol 3-Kinase/AKT-Signaling Pathway
J. Am. Soc. Nephrol., July 1, 2005; 16(7): 1936 - 1947.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
Y. K. Lee, T. Kwon, D. J. Kim, W. Huh, Y.-G. Kim, H.-Y. Oh, and H. Kawachi
Ultrastructural study on nephrin expression in experimental puromycin aminonucleoside nephrosis
Nephrol. Dial. Transplant., December 1, 2004; 19(12): 2981 - 2986.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
G. Guo, D. J. Morrison, J. D. Licht, and S. E. Quaggin
WT1 Activates a Glomerular-Specific Enhancer Identified from the Human Nephrin Gene
J. Am. Soc. Nephrol., November 1, 2004; 15(11): 2851 - 2856.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
M. Toyoda, D. Suzuki, T. Umezono, G. Uehara, M. Maruyama, M. Honma, T. Sakai, and H. Sakai
Expression of human nephrin mRNA in diabetic nephropathy
Nephrol. Dial. Transplant., February 1, 2004; 19(2): 380 - 385.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Roselli, L. Heidet, M. Sich, A. Henger, M. Kretzler, M.-C. Gubler, and C. Antignac
Early Glomerular Filtration Defect and Severe Renal Disease in Podocin-Deficient Mice
Mol. Cell. Biol., January 15, 2004; 24(2): 550 - 560.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
A. Patari, C. Forsblom, M. Havana, H. Taipale, P.-H. Groop, and H. Holthofer
Nephrinuria in Diabetic Nephropathy of Type 1 Diabetes
Diabetes, December 1, 2003; 52(12): 2969 - 2974.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. Koop, M. Eikmans, H. J. Baelde, H. Kawachi, E. de Heer, L. C. Paul, and J. A. Bruijn
Expression of Podocyte-Associated Molecules in Acquired Human Kidney Diseases
J. Am. Soc. Nephrol., August 1, 2003; 14(8): 2063 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. R. Foster, R. Hole, K. Anderson, S. C. Satchell, R. J. Coward, P. W. Mathieson, D. A. Gillatt, M. A. Saleem, D. O. Bates, and S. J. Harper
Functional evidence that vascular endothelial growth factor may act as an autocrine factor on human podocytes
Am J Physiol Renal Physiol, June 1, 2003; 284(6): F1263 - F1273.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J.-L. Michaud, L. I. Lemieux, M. Dube, B. C. Vanderhyden, S. J. Robertson, and C. R.J. Kennedy
Focal and Segmental Glomerulosclerosis in Mice with Podocyte-Specific Expression of Mutant {alpha}-Actinin-4
J. Am. Soc. Nephrol., May 1, 2003; 14(5): 1200 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
A. Suzuki, T. Ito, E. Imai, M. Yamato, H. Iwatani, H. Kawachi, and M. Hori
Retinoids Regulate the Repairing Process of the Podocytes in Puromycin Aminonucleoside-induced Nephrotic Rats
J. Am. Soc. Nephrol., April 1, 2003; 14(4): 981 - 991.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S. Doublier, G. Salvidio, E. Lupia, V. Ruotsalainen, D. Verzola, G. Deferrari, and G. Camussi
Nephrin Expression Is Reduced in Human Diabetic Nephropathy: Evidence for a Distinct Role for Glycated Albumin and Angiotensin II
Diabetes, April 1, 2003; 52(4): 1023 - 1030.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
A. Wernerson, F. Duner, E. Pettersson, S. M. Widholm, U. Berg, V. Ruotsalainen, K. Tryggvason, K. Hultenby, and M. Soderberg
Altered ultrastructural distribution of nephrin in minimal change nephrotic syndrome
Nephrol. Dial. Transplant., January 1, 2003; 18(1): 70 - 76.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
H. Pavenstadt, W. Kriz, and M. Kretzler
Cell Biology of the Glomerular Podocyte
Physiol Rev, January 1, 2003; 83(1): 253 - 307.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
D. J. Kelly, P. Aaltonen, A. J. Cox, J. R. Rumble, R. Langham, S. Panagiotopoulos, G. Jerums, H. Holthofer, and R. E. Gilbert
Expression of the slit-diaphragm protein, nephrin, in experimental diabetic nephropathy: differing effects of anti-proteinuric therapies
Nephrol. Dial. Transplant., July 1, 2002; 17(7): 1327 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
T. Palmen, S. Lehtonen, A. Ora, D. Kerjaschki, C. Antignac, E. Lehtonen, and H. Holthofer
Interaction of Endogenous Nephrin and CD2-Associated Protein in Mouse Epithelial M-1 Cell Line
J. Am. Soc. Nephrol., July 1, 2002; 13(7): 1766 - 1772.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. Rantanen, T. Palmen, A. Patari, H. Ahola, S. Lehtonen, E. Astrom, T. Floss, F. Vauti, W. Wurst, P. Ruiz, et al.
Nephrin TRAP Mice Lack Slit Diaphragms and Show Fibrotic Glomeruli and Cystic Tubular Lesions
J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1586 - 1594.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
K. Yan, J. Khoshnoodi, V. Ruotsalainen, and K. Tryggvason
N-Linked Glycosylation Is Critical for the Plasma Membrane Localization of Nephrin
J. Am. Soc. Nephrol., May 1, 2002; 13(5): 1385 - 1389.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
H. Yuan, E. Takeuchi, G. A. Taylor, M. McLaughlin, D. Brown, and D. J. Salant
Nephrin Dissociates from Actin, and Its Expression Is Reduced in Early Experimental Membranous Nephropathy
J. Am. Soc. Nephrol., April 1, 2002; 13(4): 946 - 956.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
W. Huh, D. J. Kim, M.-K. Kim, Y. G. Kim, H.-Y. Oh, V. Ruotsalainen, and K. Tryggvason
Expression of nephrin in acquired human glomerular disease
Nephrol. Dial. Transplant., March 1, 2002; 17(3): 478 - 484.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. A. Saleem, M. J. O'Hare, J. Reiser, R. J. Coward, C. D. Inward, T. Farren, C. Y. Xing, L. Ni, P. W. Mathieson, and P. Mundel
A Conditionally Immortalized Human Podocyte Cell Line Demonstrating Nephrin and Podocin Expression
J. Am. Soc. Nephrol., March 1, 2002; 13(3): 630 - 638.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
A. BENIGNI, S. TOMASONI, E. GAGLIARDINI, C. ZOJA, J. A. GRUNKEMEYER, R. KALLURI, and G. REMUZZI
Blocking Angiotensin II Synthesis/Activity Preserves Glomerular Nephrin in Rats with Severe Nephrosis
J. Am. Soc. Nephrol., May 1, 2001; 12(5): 941 - 948.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
S. Doublier, V. Ruotsalainen, G. Salvidio, E. Lupia, L. Biancone, P. G. Conaldi, P. Reponen, K. Tryggvason, and G. Camussi
Nephrin Redistribution on Podocytes Is a Potential Mechanism for Proteinuria in Patients with Primary Acquired Nephrotic Syndrome
Am. J. Pathol., May 1, 2001; 158(5): 1723 - 1731.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. PATRAKKA, V. RUOTSALAINEN, I. KETOLA, C. HOLMBERG, M. HEIKINHEIMO, K. TRYGGVASON, and H. JALANKO
Expression of Nephrin in Pediatric Kidney Diseases
J. Am. Soc. Nephrol., February 1, 2001; 12(2): 289 - 296.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.