Nephrology Dialysis Transplantation, Vol 14, Issue 3 620-626, Copyright © 1999 by Oxford University Press
M Bosomworth, S Aparicio and A Hay
Background. Although an indicator of renal tubular
dysfunction, an increased urinary N-acetyl-{beta}-D-glucosaminidase
(NAG) activity might reflect increased lysosomal activity in renal tubular
cells. Methods. Puromycin aminonucleoside (PAN) was
administered to Sprague Dawley rats to induce proteinuria. Total protein,
albumin; NAG activity and protein electrophoretic pattern were assessed in
daily urine samples for 33 days. The morphological appearance of the
kidneys was examined on days three, four, six, eight and thirty three and
the NAG isoenzyme patterns on days zero, four, eight and thirty three.
Results. Following intravenous PAN urine volume and
urine NAG activity increased significantly by day two, but returned to
normal by day four. After day four all treated animals exhibited a marked
rise in urine albumin, total protein excretion and NAG activity.
Electrophoresis showed a generalised increase in middle and high molecular
weight urine proteins from day four onwards. Protein droplets first
appeared prominent in tubular cells on day four. Peak urine NAG activity
and a change in NAG isoenzyme pattern coincided with both the peak
proteinuria and the reduction in intracellular protein and NAG droplets
(day six onwards). Conclusion. This animal model
demonstrates than an increase in lysosomal turnover and hence urine NAG
activity, occurs when increased protein is presented to the tubular cells.
Urine NAG activity is thus a measure of altered function in the renal
tubules and not simply an indicator of damage. Keywords:
functional status of renal tubules; puromycin aminonucleoside;
rats; tubular damage; urine N-acetyl-{beta}-D-glucosaminidase
ORIGINAL ARTICLES
Urine N-acetyl-{beta}-D-glucosaminidase - a marker of tubular damage?
Chemical Pathology, Histopathology, St James's University Hospital, Beckett Street, Leeds LS9 7TF, UK; Department of Chemical Pathology and Immunology, Leeds General Infirmary, Leeds, UK; Corresponding author
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