Nephrology Dialysis Transplantation, Vol 14, Issue 3 581-587, Copyright © 1999 by Oxford University Press
M Bek, K Fischer, S Greiber, C Hupfer, P Mundel and H Pavenstadt
Background. Dopamine influences glomerular
haemodynamics and dopamine receptors have been demonstrated in the
glomerulus, but little is known about the cellular effects of dopamine in
glomerular cells. The aim of this study was to investigate the influence of
dopamine on the cellular functions of podocytes. Methods.
The effect of dopamine on membrane voltage was investigated in
differentiated mouse podocytes. The membrane voltage was measured using the
patch clamp technique. Reverse transcribed-polymerase chain reaction
(RT-PCR) studies were performed to investigate the expression of dopamine
receptor mRNA in mouse glomeruli and podocytes. Results.
The addition of dopamine (100 nM-1000 {mu}M) caused a
concentration-dependent depolarization of podocytes (EC50≈10 {mu}M).
Like dopamine, the selective agonist of the D1-like receptor, SKF 82958,
depolarized podocytes in a concentration-dependent manner. (EC50≈50
{mu}M). SKF 82958 stimulated a time-and concentration-dependent
accumulation of cyclic adenosine 3′,5′-monophosphate
(cAMP) in podocytes (EC50≈5 &mgr;M). RT-PCR studies with primers
derived from mouse sequences amplified mouse mRNA for the D1-like and the
D2-like receptor in glomeruli, which were obtained by the sieve technique,
whereas only mRNA for the D1-like receptor was detected in cultured mouse
podocytes. Conclusion. The data indicate that dopamine
induces a cAMP-dependent depolarization via a D1-like receptor in
podocytes. Keywords: cAMP; dopamine; dopamine
receptors; membrane voltage; podocyte
ORIGINAL ARTICLES
Dopamine depolarizes podocytes via a D1-like receptor
Department of Medicine, Division of Nephrology, University of Freiburg, Freiburg, Germany; Department of Anatomy and Cell Biology, University of Heidelberg, Heidelberg, Germany; Corresponding author address: Medizinische Universitatsklinik, Abt. IV, Labor C4, Hugstetter Str. 55, D-79106 Freiburg, Germany
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