Nephrol Dial Transplant (1999) 14: 2413-2419
© 1999 European Renal Association-European Dialysis and Transplant Association
PDGF-AB release during and after haemodialysis procedure
Institute of Nephrology, St. Orsola University Hospital, Bologna, Italy
Correspondence and offprint requests to: Dr Giuseppe Cianciolo, Servizio di Nefrologia e Dialisi, Policlinico S. Orsola, Via Massarenti 9, I-40138 Bologna, Italy.
Background. During haemodialysis blood–membrane contact causes the release of the content of platelet 
-granules, which contain platelet-derived growth factor (PDGF). In view of its possible role in accelerated atherosclerotic processes, we evaluated the intra- and post-dialytic changes in PDGF-AB serum levels during haemodialysis sessions performed using a cellulosic membrane.
Methods. Using the ELISA method, PDGF-AB, platelet factor-4 (PF4) and ß-thromboglobulin (ß-TG) levels were determined in peripheral blood, as well as in arterial and venous haemodialyser lines, in 10 patients each of whom underwent five consecutive dialysis sessions with a CU membrane. Blood samples were taken at 0, 15, 30, 60, 120, 180 and 240 min during dialysis and at 1, 4 and 20 h after the end of the session. In the same group of patients the levels of the same molecules were also determined after a heparin bolus injection of 4500 IU, blood samples were taken at 0, 15 and 30 min after injection of the bolus.
Results. PDGF-AB serum levels increased, remained consistently high during the haemodialysis session (in particular +134±20% after 30 min, P<0.001, and +140±5% after 240 min, P<0.001) and returned to basal values only after 20 h following the end of the session. PF4 and ß-TG showed a similar trend to PDGF. The heparin bolus injection caused only a small increase (+15±5% at 30 min) in PDGF-AB serum levels.
Conclusions. PDGF-AB is released during dialysis mainly as consequence of the blood–membrane contact and it returns only slowly to basal values.
Keywords: accelerated atherosclerosis; dialysis; growth factors; platelet activation; platelet-derived growth factor
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