Nephrology Dialysis Transplantation, Vol 14, Issue 1 76-80, Copyright © 1999 by Oxford University Press
T Wada, K Furuichi, N Sakai, M Shimizu, C Segawa, K Kobayashi, N Mukaida, T Kasahara, K Matsushima and H Yokoyama
Background: A potent eosinophil chemotactic cytokine,
human eotaxin, is directly chemotactic for eosinophils. Therefore, the
specific expression of eotaxin in tissue might play a crucial role in
tissue eosinophilia. However, the precise molecular mechanism of the
recruitment and activation of eosinophils in human renal diseases remains
to be investigated. We evaluated the role of eotaxin in the pathogenesis of
human diffuse interstitial nephritis with marked infiltration of
eosinophils. Methods: In this study, we examined 20
healthy volunteers, 56 patients with primary or secondary glomerular
diseases and two hypereosinophilic syndrome patients without renal
involvement. Urinary and serum eotaxin levels were determined by an
enzyme-linked immunosorbent assay. We also detected the presence of eotaxin
protein immunohistochemically. Results: On the one
hand, urinary levels of eotaxin were significantly higher before the
initiation of glucocorticoid administration in the patient with
interstitial nephritis with marked infiltration of eosinophils. On the
other hand, urinary eotaxin levels were not detected in any patients with
nephrotic syndrome, interstitial nephritis without eosinophils,
hypereosinophilic syndrome without renal involvement or other renal
diseases. Serum eotaxin levels were not detected in any of the patients.
Therefore, the detection of eotaxin in the urine was specific for renal
interstitial eosinophilia. Moreover, endothelial cells, infiltrating
mononuclear cells and renal epithelial cells in the tubulointerstitial
lesions were immunostained with specific anti-eotaxin antibodies.
Furthermore, the elevated urinary levels of eotaxin decreased dramatically
during glucocorticoid-induced convalescence.
Hypothesis: We hypothesize that in
situ expression of eotaxin may provide a new mechanism to
explain the renal interstitial eosinophil infiltration. Key
words: chemokine; eosinophilia; eotaxin; interstitium; renal
disease
ORIGINAL ARTICLES
Eotaxin contributes to renal interstitial eosinophilia
First Department of Internal Medicine and Division of Blood Purification, School of Medicine, Department of Molecular Pharmacology, Cancer Research Institute, Kanazawa University, Kanazawa, Japan; Division of Biochemistry, Kyouritsu College of Pharmacy, and Department of Molecular Preventive Medicine, University of Tokyo, Tokyo, Japan; Corresponding author at: First Department of Internal Medicine, Kanazawa University School of Medicine, 13-1 Takara-machi, Kanazawa 920-8641, Japan
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