Nephrology Dialysis Transplantation, Vol 13, Issue 9 2288-2293, Copyright © 1998 by Oxford University Press
S Cantaro, G Zara, C Battaggia, P di Loreto, L Calo, A D'Angelo and G Theophidlidis
Background: In order to investigate the aetiology of
uraemic neuropathy, we evaluated the neurotoxic activity of plasma from
uraemic patients. To this end we prepared a concentrate (1 : 1000) of 2-60
kDa MW compounds from paired filtration dialysis ultrafiltrate and
evaluated its activity on peripheral nerve conduction in
vivo and in vitro.
Methods: The in vivo
neurotoxicity was tested on rat sciatic nerve by intraneural injection of
the uraemic concentrate, followed, 1 to 6 days later, by
electrophysiological assessment of motor response and maximum conduction
velocity. In vitro experiments were performed on
isolated frog sciatic nerve in the presence of uraemic concentrate, and the
neurotoxicity was evaluated from the rate of the decrease in the amplitude
of the evoked maximal action potential. Results: In
the in vivo experiments, the sciatic nerves injected
with the uraemic concentrate showed a decrease in maximum conduction
velocity and a progressive impairment in evoked motor response. In the
in vitro experiments uraemic concentrate induced a
dose-dependent neurotoxic effect. Conclusions: Our
study demonstrates the presence in plasma of uraemic patients of a compound
of 2-60 kDa MW with neurotoxic activity. Key words:
uraemia; neuropathy; dialysis; polysulphone membranes
ORIGINAL ARTICLES
In vivo and in vitro neurotoxic action of plasma ultrafiltrate from uraemic patients
Institute of Internal Medicine, Division of Nephrology 1, Department of Neurological and Psychiatric Sciences, University of Padova, Italy; Department of Neurotraumatology, Giustiniani Hospital, Padova,, Italy; Laboratory of Animal Physiology, Department of Zoology, Aristotle University, Thessaloniki, Greece; Corresponding author at: Istituto di Medicina Interna, Divisione di Nefrologia 1, via Giustiniani 2, I-35128 Padova, Italy
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