Nephrology Dialysis Transplantation, Vol 13, Issue 6 1446-1451, Copyright © 1998 by Oxford University Press
M Pahl, F Oveisi, G Khamiseh and N Vaziri
Background: Nephrotic syndrome (NS) results in
hypercholesterolemia which is attributed to increased production and
decreased removal of cholesterol-rich lipoproteins. Adjustments in
intestinal absorption are reportedly involved in cholesterol homeostatis.
We therefore, studied the intestinal absorption and biliary excretion of
cholesterol in NS. Methods: We studied intestinal
absorption (by in vivo perfusion and in
vitro everted sac incubation techniques) and biliary secretion
(by common bile duct cannulation) of cholesterol in rats with
puromycin-induced NS. The results were compared with those obtained from
pair-fed control (PF) animals, those given free access to food (NL) or
those fed a hypercholerolemic diet (H-chol group). Micellar solutions of
Krebs' phosphate buffer containing trace amounts of
[14C]inulin and
[3H]cholesterol, as well as different concentrations
of unlabeled cholesterol, were used for absorption studies.
Results: The NS and H-chol groups showed severe and
comparable hypercholesterolemia. No significant difference was found in the
rate of biliary cholesterol secretion among the study groups. Likewise, the
rates of in vivo and in vitro
cholesterol absorptions in the NS and H-chol groups were comparable with
one another and similar to those found in the NL and PF groups. The rate of
in vitro cholesterol absorption was directly
proportional to its concentration in the incubation media at low
concentrations. However, the absorption rate showed a pattern consistent
with saturable transport at high cholesterol concentrations in all groups.
Conclusions: We conclude that intestinal absorption
and biliary secretion of cholesterol are not appreciably influenced by
either nephrotic or diet-induced hypercholesterolemia in rats. The data
further suggest that cholesterol absorption may be a saturable process.
Key words: biliary secretion; cholesterol absorption;
hypercholesterolemia; hyperlipidemia; intestinal absorption; nephrotic
syndrome; proteinuria
ORIGINAL ARTICLES
Intestinal absorption and biliary secretion of cholesterol in rats with nephrotic syndrome
Division of Nephrology, UCI Medical Center, 101 The City Drive, Orange, CA 92868, USA; Corresponding author
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