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Nephrology Dialysis Transplantation, Vol 13, Issue 5 1211-1214, Copyright © 1998 by Oxford University Press

ORIGINAL ARTICLES

A 3-year follow-up of HCV-RNA viraemia in haemodialysis patients

F Galan, M Perez-Gracia, A Lozano, B Benavides, E Fernandez-Ruiz and M Rodriguez-Iglesias
Laboratory of Microbiology and Haemodialysis Unit, Puerto Real University Hospital, CN IV, Km 665, E-11510-Puerto Real, Cádiz, Spain; Corresponding author

Background: Hepatitis C virus (HCV) infection in a population of haemodialysed patients was studied over a 3-year follow-up period in order to evaluate the changes in viral RNA, diversity of genotypes, and serological response to synthetic HCV peptides. Methods: Twenty-eight (32.9%) patients with anti-HCV antibodies from a total of 85 patients assigned to a haemodialysis unit were studied. The serological response to immunopetides was evaluated by immunoblotting, viral RNA in serum was detected using the polymerase chain reaction (RT-PCR), and genotyping was carried out by hybridization with probes fixed to nitrocellulose paper. Results: Of the 28 haemodialysis patients who had anti-HCV antibodies, three (10.7%) were always RNA negative, six (21.4%) were always RNA positive, and 19 (67.8%) were variable RNA. There was an incomplete antibody response to nonstructural antigens in non-viraemic patients. Genotype was determined in 23 patients, and the other two could not be genotyped. The most common genotype was 1b (69.4%), followed by 1a (17.4%), and 2a 3a, and 4a (each 4.4%). Conclusions: Haemodialysis patients, when followed up for a long time, frequently show an intermittent HCV viraemia state, suggesting that HCV cannot be evaluated adequately by isolated RNA determinations. Key words: anti-HCV antibodies; genotypes; haemodialysis; hepatitis C infection; hepatitis C virus
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