Nephrology Dialysis Transplantation, Vol 13, Issue 11 2821-2824, Copyright © 1998 by Oxford University Press
M Zhao, N Liu, Y Zhang and H Wang
Background: ANCA have been found in patients with
systemic lupus erythematosus (SLE); however, the prevalence of ANCA and
their target antigens is still not certain. This study is to investigate
the prevalence of ANCA and their target antigens in Chinese patients with
lupus nephritis. Methods: Ninety-five serum samples
were collected from 95 renal-biopsy-proven lupus nephritis patients.
Indirect immunofluorescence using ethanol-fixed leukocytes as substrate and
ELISA using six highly purified known ANCA antigens as solid-phase ligands
were performed. The specific ANCA antigens included proteinase 3,
myeloperoxidase, bactericidal/permeability-increasing protein, human
leukocyte elastase, cathepsin G, and lactoferrin. The prevalence of ANCA in
patients with (n=65) and without (n=30) active renal pathological lesions
was also compared to reveal whether ANCA correlates with disease activity.
Results: (i) None of the sera recognized proteinase 3,
myeloperoxidase, and human leukocyte elastase, and only one serum
recognized bactericidal/permeability-increasing protein. The striking
finding was that 59/95 (62.1%) sera recognized cathepsin G and the titres
of some sera reached 1/3200. Eight of 95 sera (8.4%) recognized
lactoferrin. (ii) The percentage of anticathepsin G antibody positive
samples in patients with active renal lesions was significantly higher than
in patients without active lesions (73.4 vs 36.7%,
P<0.0001), whereas, anti-lactoferrin antibodies had no correlation
with active renal lesions. (iii) By indirect immunofluorescence, only 22%
of the 95 sera were ANCA positive. Conclusions: Our
results suggest that the majority of lupus nephritis patients have ANCA and
that the major target antigens is cathepsin G. Anti-cathepsin G antibodies
seem to be correlated with renal disease activity. Key
words: ANCA; autoantibodies; autoantigen; autoimmune disease;
cathepsin G; lupus nephritis; SLE; vasculitis
ORIGINAL ARTICLES
Antineutrophil cytoplasmic autoantibodies (ANCA) and their target antigens in Chinese patients with lupus nephritis
Department of Nephrology, 1st Teaching Hospital, Beijing Medical University, Beijing 100034, China; Corresponding author
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