Nephrology Dialysis Transplantation, Vol 13, Issue 10 2588-2593, Copyright © 1998 by Oxford University Press
V Schettler, E Wieland, H Methe, P Schuff-Werner and G Muller
Background. Living cells are protected by free radical
scavenging enzymes against oxygen radical-mediated damage. It has been
suggested that granulocytes are activated on the surface of dialyser
membranes, resulting in the generation of free radicals. We have recently
reported a lack of plasma lipid peroxidation and unchanged glutathione
peroxidase (GSH-Px) as well as glutathione reductase (GSSG-R) activities in
red blood cells of haemodialysis patients. However, because mature red
cells are free of DNA and RNA, free radical scavenging enzymes (FRSE)
cannot be regulated on the gene level in response to an acute oxidative
stress. In contrast to erythrocytes, granulocytes are nucleated cells and
FRSE protein concentrations can therefore be modulated. Methods.
GSH-Px, GSSG-R, superoxide dismutase (SOD) activities and total
glutathione (GSH) were determined spectrophotometrically using a Cobas Fara
semi-automatic analyser in granulocytes of 31 healthy blood donors and in
28 patients with chronic renal failure (CRF) for more than 6 months before
as well as immediately after a single dialysis treatment. Patients were
treated either by haemodialysis (n=17) using low-flux
polysulphone membranes or by haemofiltration (n=11)
using high-flux polysulphone membranes. Results.
Compared to healthy controls, SOD and GSSG-R activities were
increased in granulocytes of HD and HF patients, GSH and GSH-Px were
decreased before a single treatment. After dialysis SOD and GSH-PX
activities were significantly induced by both HD and HF whereas GSSG-R
activities and GSH were decreased. Conclusions. These
results show that the enzymatic defence against oxygen radicals can be
induced in granulocytes of patients undergoing regular dialysis treatment,
whereas the non-enzymatic defence is compromised as shown by decreased GSH
concentrations, both suggesting increased oxidative stress.
Keywords: extracorporeal circulation; free radical
scavenging enzyme activity; granulocyte; haemofiltration; haemodialysis;
oxygen radicals
ORIGINAL ARTICLES
Oxidative stress during dialysis: effect on free radical scavenging enzyme (FRSE) activities and glutathione (GSH) concentration in granulocytes
Department of Nephrology and Rheumatology, Department of Clinical Chemistry, Georg-August-Universitat Gottingen, Zentrum Innere Medizin, Robert-Koch-Str. 40, D-37070 Gottingen, Germany; Corresponding author
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