Nephrology Dialysis Transplantation, Vol 13, Issue 10 2529-2535, Copyright © 1998 by Oxford University Press
M Herten, W Lenz, R Gerzer and C Drummer
Background. The natriuretic peptide urodilatin was
first isolated from human urine and may be one of the important mediators
of natriuresis, while the atrial natriuretic peptide &agr;-ANP, the
circulating member of the family, rather seems to play a role in
cardiovascular regulation. Although the renal expression of the common
propeptide for urodilatin and &agr;-ANP has been detected in rat and
pig, it is not yet shown that urodilatin is synthesized in human kidney.
Methods. Immunohistochemically we localized urodilatin
with an urodilatin-antibody, which does not cross-react with &agr;-ANP,
the ANP propeptide, brain natriuretic peptide (BNP), and C-type natriuretic
peptide (CNP). Radiolabelled urodilatin binding was examined
autoradiographically. Results. We could demonstrate
that urodilatin is present in human distal tubular cells, in which we could
also localize propeptide immunoreactivity. The glomeruli, the cells of the
proximal tubule, and the collecting duct did not show any urodilatin
immunoreactivity. In human kidney homogenate the urodilatin content was
4600±520 fmol/g protein (n=6). The renal
concentration of BNP and CNP, mainly localized in the distal tubule, was
much lower at 40±8 and 400±50 fmol/g protein
(n=6) respectively. Furthermore the autoradiographic
examinations showed that radiolabelled urodilatin binds to natriuretic
peptide receptors in the glomeruli, blood vessels, and collecting ducts.
Conclusions. Our data suggest that urodilatin may be
the predominant representative of natriuretic peptides in human kidneys.
Urodilatin being synthesized in the distal tubular region may be
transported as a paracrine factor to the collecting duct, where it exerts
its suppressing effect on the sodium reabsorption by stimulating the
guanylyl cyclase A. Keywords: autoradiography;
immunhistochemistry; natriuretic peptides
ORIGINAL ARTICLES
The renal natriuretic peptide urodilatin is present in human kidney
Institut fur Luft- und Raumfahrtmedizin, Deutsches Forschungszentrum fur Luft und Raumfahrt (DLR), D-51170 Koln, Germany; Corresponding author
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