Nephrology Dialysis Transplantation, Vol 12, Issue 12 2537-2541, Copyright © 1997 by Oxford University Press
S Harendza, U Behrens, G Zahner, A Schneider and R Stahl
Background: Mesangial cell proliferation is a
predominant feature of many glomerular diseases. We have demonstrated in
previous studies that an experimental model of mesangial-proliferative
glomerulonephritis in the rat could be transferred to in
vitro conditions. Using this model we now have been able to
study the mesangial phenotype in several subcultures of mesangial cells
from nephritic animals regarding proliferation, and the synthesis of
prostaglandins and the matrix degrading enzyme MMP-2.
Methods: Mesangial proliferative glomerulonephritis
was induced in male Sprague-Dawley rats by a single injection of an
anti-Thy 1.1 antiserum. Four days after injection of the antiserum
glomeruli were isolated and transferred to tissue culture conditions.
Immunohistological characterization of cells, cell growth, synthesis of
prostaglandins and expression of MMP-2 were studied in the first and second
subculture. Results: Cells from controls and from
nephritic animals showed the characteristics of glomerular mesangial cells.
Proliferation was decreased in the first and second subcultures of cells
from nephritic rats compared with controls while there were no
immunohistological differences between the cells. Biosynthesis of
prostaglandin E2 was significantly increased in subcultures of mesangial
cells from nephritic rats. There was also a significant increase in mRNA
expression of MMP-2 in the first subculture of mesangial cells from
nephritic rats when compared with controls.
Conclusions: Our data suggest that mesangial cells
from nephritic animals show a different phenotype in
vitro in several subcultures compared with cells from control
animals. This difference can be demonstrated in the patterns of
proliferation and biosynthesis of prostaglandins and MMP-2, while
immunohistological characteristics of mesangial cells were unchanged
between nephritic animals and controls. This experimental in
vivo-in vitro approach may serve as a model to study the
mesangial phenotype in glomerular disease. Key words:
glomerulonephritis; mesangial phenotype; metalloproteinases; prostaglandins
ORIGINAL ARTICLES
In vitro characterization of the mesangial phenotype in a proliferative glomerulonephritis of the rat
Department of Medicine, Division of Nephrology and Osteology, University Hospital Eppendorf, Hamburg, Germany; Corresponding author at: Universitatskrankenhaus Eppendorf, Medizinsche Klinik, Abt. Nephrologie/Osteologie, Pavillon 61, Martinstr. 52, D-20246 Hamburg, Germany
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